丁苯酞动员内皮祖细胞治疗缺血性卒中大鼠的实验分析

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1、丁苯酞动员内皮祖细胞治疗缺血性卒中大鼠的实验研究英文摘要ExperimentalstudyonButylphthalidetreatingischemicstrokebymobilizationofendothelialprogenitorcellsAbstractObjective:ToexploretheefficacyandmechanismofButylphthalideonischemicstroke,effectofdifferentdosesofButylphthalide(NBP)ontheneurologicalfunction,circulatingendoth

2、elialprogenitorcells(EPCs)andneurogenesisintheischemiaregionwereobservedinmiddlecerebralarteryocclusion(MCAO)/reperfusionrat.Method:48maleSprague-Dawleyratsweighted(250±20)gwereselectedinthisstudy,whichwererandomlydividedintofourgroups,ie.shamgroup,modelcontrolgroup,lowdoseNBPgroupandhighdoseN

3、BPgroup,and12animalsineachgroup.Neckvesselswereisolatedbutthreadembolismwasnotinsertedinshamgroup.Ontheotherthreegroups,threadembolismmethodwasusedtopreparemiddlecerebralarteryocclusion(MCAO)/reperfusionanimalmodel.TworatsinbothshamgroupandmodelcontrolgroupwereperformedTTCstainingtoobservemorp

4、hologicalchangesinischemicarea.TherestratswererespectivelygavagedwithtwodosesofNBPorcontrolplacebo(soybeanoil)(2ml/d)for5days.Neurologicalfunctionofallratswereassessedeveryday.PeripheralbloodwascollectedbeforeandaftertreatmenttoinvestigatethelevelofcirculatingEPCswithflowcytometry.Immunochemic

5、alstainingofCD31andvWF(markersofvascularendothelialcells)wasperformedtoobserveangiogenesisafter5daysoftreatment.AllthedatawereanalyzedbyGraphPad5.0softwareanalysissystem.Results：1）Hemisphereonthesideofcarotidarteryinsertedwiththreadembolismdisplayedpalecolorandhemisphereontheothersidedisplayed

6、redcolor.2）Onday1aftertreatment,neurologicalfunctionscorereducedinlowdoseNBPgroup(P<0.05),anditsignificantlyreducedinhighdosegroup(P<0.01).Thescorewassignificantlydecreasedonday3aftertreatmentinmodelcontrolgroup.3）Onday1aftertreatment,neurologicalfunctionscoreinlowdoseNBPgroupwereIII英文摘要丁苯酞动员内

7、皮祖细胞治疗缺血性卒中大鼠的实验研究lowerthanthecontrolgroup(P<0.05),thescoreinhighdosegroupwassignificantlylowerthanthecontrolgroup(P<0.01);Onday2-5aftertreatment,thescoreofdifferentdoseNBPgroupsweresignificantlylowerthanthecontrolgroup(P<0.01);4）Onday3