estazolam逆转结节乳头体核组胺引发的觉醒

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1、conditions．Theywer‘ehousedinindividualcagesandmaintainedunderstandardlaboratoryconditions：a．12hlight--darkcycle(1ightonat08：00am，andturnoffat20：00pro)，anambienttemperatureof24±1"C，foodandwateravailablefreely·Forthecontrolgroup，abasicsleep-wakecycleascontrolofEEGandEMGwererecordedandeve

2、ryphaseanalyzedfor24h．Forthedrug—treatedgroups，thedrugswerealldilutedbYthephysiologicsaline．Forthesalinegroup，0．5∥LphysiologicsalinewasmicroinjectedintoTMn，andfortheHAgroup，0．5pLHAwasmicfoinjectedint011矗．TheEstazolamgroupwasgivenEstazolamO，o5mg。k91，Sigma)．CorticalEEGandEMGsignalsweream

3、plified，digitizedwitharesolutionof100Hz，andcomputedonaCEDmicrol401mkII(CED，Cambridge，UK)·UsingaSpike2script，polygraphicrecordswerevisuallyscoredby30secepochsforwakefulnessandsleepstatesincludinglightslowwavesleep(SWSl)，deepslowwavesleep(sws2)andparadoxicalsleep(PS)．Thesestageswerestati

4、sticallycalculatedper30sepochsfor8h，whensalineanddrugsweresubsequentlyadministratedaccordingtoprotoc01．Thesignalswererecordedat8：00ameveryday,andrecordedcontinuousEEG／EMGsignalsfor24h．ResultsThesalinegrouphadnoeffectonthesleep-wakefulnesscycle·Microinje虻'tionHA(1』c,g-kg-1)intoTMBincrea

5、sedthedurationaltimeofwake妒<0．01)andreducedtheSWStime，butdidnoteffectonPScomparedtocontrolfor24h．Estazolam(5nag·k岔1，p．o)increasedthetotalsleeptimeinespecialincreaseofSWS2time俨

6、1．Histaminergicneuronsinthe。TMnplayakeyroleofpromotingandmaintainingthewakefulness，andinhibitedtheSWS，butnoeffectonPS．2．EstazolaminducesallincreaseofthetotalsleeptimemainlyviaincreasingofSWS2．3．EstazolamcanreverseHA-inducedwakefulnessintosleep．Keywordssleep—wakefulnesscycle；tuberomammi

7、llarynucleus；histaminergicneurons；Estazolam；GABAergicneurons英文缩写英文名英文缩略词GABAGamma—aminobutyricacidHAHistaminedihydroctdoride中文名丫一氨基丁酸组胺二盐酸组胺NREMSNon．rapideyemovementsleep非快速眼动睡眠PSParadoxicalsleepREMSRaploeyemovementsleepSWSlLightslowwavesleepSWS2DeepslowwavesleepTMnTuberomammillarynu