xaf1在胃肠道肿瘤中的表达及其在大肠癌细胞中作用的初步研究

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1、ABSTRACTofXAFlA，XAFlB，XAFlCwereincreasedbyIFN-ainMGC803cellline；2TherewasnodifferenceexpressionofXAF1Aintumorandnormaltissues(P>O．05)，theexpressionofXAF1BandXAF1Cintumorandadenomatissueswerelowthanthenormaltissue，westernblowingindicatedthat，intumor，theexpressionofXAF

2、1Awaslowcomparedtoitscorrespnormaltissue，butthedifferenceWasnotsignificant(P>0．05)；3ImmunohistochemistryAssayindicatedthatcomparedtothenormalandhyperplasytissues，inadenomaandtumortissues，XAF1nucleusstainingwasdepressed(P<0．05)，cytolymphstainingWasincreased(P<0．05)，th

3、etotalstainingWasdepressed(P

4、torsused；5LuciferaseReporterAssayindicatedthatover-expressionofXAF1suppressedtheactivityofsurvivinandcateninpromotor(P<0．10)；6AllofXAFlA，XAFlB，XAFlCwereincreasedbyIFN-ainMGC803cellline，over-expressionofXAF1doesnotinfluencetheexpressionofE-cadherin，VillinandPan．CEA．Co

5、nclusions1XAF1splicevariantsl，2and3expressionswereeitherabsentoratlowlevelsintumorcelllinesandnormaltransformatedcellline；2ThereWasnodifferenceexpressionofXAF1Aintumorandnormaltissues，thedisorderlyexpressionofXAF1BandXAF1CintumormayeffectthefunctionofXAFlA；3Innormalt

6、issue，XAF1stainingwasmaininthenucleus，whileintumor硕士学位论文tissue，XAF1stainingwasmaininthecytolymph，andthatXAF1mayplaydifferentroleintumorcellsowingtoitslocation；4over-expresisonofXAF1suppressedcancercellgrowth；5over-expressionofXAF1suppressedtranscriptionofsurvivinandc

7、atenin；6AllofXAFlA，XAFlB，XAFlCwereincreasedbyIFN-ainMGC803cellline，over-expressionofXAF1doesnotinducecancercelldifferentiation．KEYWORDS：HSXIAPAFIprotein，human；IntestinalNeoplasms；Genes，TumorSuppressor；Apoptosis；CellDifferentiationV原创性声明南方医科大学学位论文原创性声明本人郑重声明：所呈交的论文是本人

8、在导师的指导下独立进行研究所取得的研究成果。除了文中特别加以标注引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写的成果作品。对本文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明。除与外单位合作项目将予以明确方式规定外，本研究已发表与未发表成果