应用issr分子标记的研究崇州枇杷茶和荥经枇杷茶种质资源遗传多样性与亲缘关系

《应用issr分子标记的研究崇州枇杷茶和荥经枇杷茶种质资源遗传多样性与亲缘关系》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据四川农业大学硕士学位论文containsBaimaotea,Camelliaptilophylla．AccordingtoChenLiang’Sclassificationsystemofclassificationoftea，Chongzhoutea，loquatbelongstothebaldrealteaandtea，whichcontainsc．gymnogynaandBaimaotea；andYingjingloquatteaalsobelongstothebaldrealteaandtea，teaisalsocontainedinth

2、ePu’erTeaandBaimaoteatea．ChongzhouloquatteaingeneticdiversitythantheYingjingloquattea．(2)Outof60ISSRprimersselectedwereobtained14highpolymorphicprimers，primersforeachsamplewasamplifiedbyISSP-PCR，Canbeamplifiedbandswereclearandrichpolymorphism．14primerswereamplified239clearbandsCa

3、nberepeated，inwhich209bandswerepolymorphic，thepercentageofpolymorphiclociwas86．67％．Eachprimeramplifiedbandsof12-23，theaveragenumberofamplifiedbandswas17．07．(3)AccordingtotheelectrophoresisresultsofISSRamplificationproducts，theuseofNei’Smethodtocalculatetherangeofvariationof45acce

4、ssionsofChongzhouloquatteageneticdistancebetweenthetestedmaterialswasbetween0．594～0．858，thegeneticdistancebetweenC34andC38recently，0．594，andgeneticdistancebetweenC42andC23isthe0．858．AccordingtothegeneticsimilaritycoefficientclusteraccordingtothemethodofUPGMA，itCanbeseenfromthefig

5、ure，whentheGSvalueisO．705．ChongzhouloquatteaCanbedividedinto6categories．(4)AccordingtotheelectrophoresisresultsofISSRamplificationproducts，theuseofNei’Smethodtocalculatethe22testedmaterialsYingjingloquatteageneticsimilaritycoefficientbetweentheGSvalueof0．598-0．774．Amongthem，Y14an

6、dY18themaximumGSvaluewas0．774，thegeneticrelationship，recently．TheminimumGSvalueisY16andY4，theGSvalueisO．598，indicatingthatthegeneticrelationshipbetweenthefarthest．AccordingtothegeneticsimilaritycoefficientclusteraccordingtothemethodofUPGMA，itCanbeseenfromthefigure，whenthegeneticd

7、istancewas0．703，22copiesofYingjingloquatteacanbedividedintofivecategories．(5)Accordingtotheclusteranalysisof75accessions，resultsshowthatthegeneticsimilaritycoefficientbetweendifferentmaterialsin0．552～0．857．WhentheGSvalueisO．650，thematerialscanbedividedinto2majorgroups．WhentheGSIV

8、万方数据四川农业大学硕士学位论文valueis0．688，67accession