动态高压微射流技术对牛血清白蛋白糖基化理化性质和结构的影响

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1、响，对同一压力预处理不同反应时间下的蛋白质糖基化产物的功能性质和结构进行测定。结果表明：DHPM能明显促进BSA-乳糖糖基化反应终产物的生成；BSA-葡聚糖糖基化产物的起泡性能显著提高，经DHPM预处理的BSA-葡聚糖糖基化产物的起泡性能进一步改善，而其乳化性能却降低，但总体高于原样；DHPM对BSA-葡聚糖糖基化产物的热稳定起负面作用；红外图谱表明BSA与葡聚糖发生共价交联；近紫外圆二色谱表明BSA-葡聚糖糖基化后造成蛋白分子三级结构的变化，经DHPM处理的BSA葡聚糖糖基化产物与未经压力处理的有所差别。关键词：动态高

2、压微射流；牛血清白蛋白；糖基化；理化性质；结构特性IIAbstractWiththedevelopmentofeconomyandtheimprovementofpeople'slivingstandards,manynaturalproteinfunctionalpropertiescan'tmeettherequirementsoffoodproductionandconsumption,functionalpropertiesofproteininthefoodprocessingandstorageprocess

3、playsaveryimportantrole.Therefore,improvethefunctionalpropertiesofproteintosatisfytheneedsoffoodproductionandconsumptionhasbecomeagoalbymanyfoodscienceworkers.Glycosylationreactionisagoodwaytoimproveproteinfunctionalpropertiesofprotein.Itcanalsogivenewfeaturesatt

4、hesametime.Inaddition,theearlystageoftheresearchstudyshowsthatdynamichighpressuremicrofluidizationcanpromote‘glycosylationreactionofprotein.Therefore,theglucose,lactose,dextranandbovineserumalbumin(BSA)wereastherawmaterialinthispaper,weresearchedontheBSA-glucose,B

5、SA-lactoseandBSA-dextransystemglycosylationreactioneffectofpretreatmentbydynamichighpressuremicrofluidization(DHPM),andtheglycosylationproteinfunctionalpropertiesandstructureofthemutualconnectiontoprovidetheoreticalbasisforDHPMpromotesproteinglycosylationreactiona

6、ndcertaintechnicalreferenceinthefoodfieldformodificationofprotein.Themainresearchcontentsandconclusionsareasfollows:(1)WithBSAandglucoseasrawmaterial,weresearchedtheeffectofprotein-monosaccharideglycosylationreactionbyDHPMpretreatmentanddetermineedthefunctionalpr

7、opertiesandstructureoftheproteinbeforeandafterthereaction.ResultsshowedthatDHPMcouldobviouslypromotetheproductionofintermediateandfinalproductsofBSA-glucoseglycosylationreaction,andincreasethedegreeofglycosylationreaction.AfterDHPMtreatment,thecontentoffreeaminog

8、roupofBSA-glucoseglycosylationproductsignificantlyreduced,andfirstdecreasedthenincreasedwiththepressureincreasing,thelowestat120MPa.TheperformanceoffoamingofBS