usethefollowingtoanswerquestions1-10：使用下面的回答问题

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1、8Chapter9CatalyticStrategiesChapter9CatalyticStrategiesMatchingQuestionsUsethefollowingtoanswerquestions1-10:Choosethecorrectanswerfromthelistbelow.Notalloftheanswerswillbeused.a)hydrolysisb)stopped-flowc)site-directedmutagenesisd)chymotrypsine)zincf)stereochemicalmonitoringg)magnesium

2、h)two-foldrotationali)methylationj)peptidebondcleavage____________Anenzymethattemporarilyundergoescovalentcatalysisaspartofitsmechanism.Ans:dSection:  9.1____________Thetypeofreactioncatalyzedbyproteases.Ans:aSection:  9.1____________Themetalionrequiredbycarbonicanhydraseforactivity.An

3、s:eSection:  9.2____________Theprocessbywhichchymotrypsinogenisconvertedintoactivechymotrypsin.Ans:jSection:  9.1____________Atechniquethatpermitsrapidmonitoringofenzymekinetics.Ans:bSection:  9.1____________TheprocessbywhichhostDNAisprotectedfromcleavagebythehostrestrictionendonulease

4、s.Ans:iSection:  9.38Chapter9CatalyticStrategies____________Atechniquethataidsindeterminationofstructure/functionrelationshipsinenzymes.Ans:cSection:  9.1____________Themetalionfrequentlyfoundatactivesitescontainingphosphategroups.Ans:gSection:  9.3____________Atechniquethatcanbeusedto

5、determinemechanismswhenchiralmoleculesareinvolvedinreactions.Ans:fSection:  9.3____________Invertedrepeatsindouble-strandedDNAcreatethistypeofsymmetry.Ans:hSection:  9.3FillintheBlankQuestionsForaproteaseinhibitortobeeffective,itmustbe_________________foroneenzyme.Ans:specificSection:9

6、.1Thecatalyticmechanismofadenylatekinase,inwhichthesubstratesareorientedtostabilizethetransitionstate,iscalled___________________.Ans:catalysisbyapproximationSection:9.4A-Tbasepairsareeasilyinterrupted,astheycontainonly_____hydrogenbondsversus_______hydrogenbondsfoundinG-Cbasepairs.Ans

7、:two,threeSection:9.3Themechanismofchymotrypsininvolvestheformationofanunstable__________________-shapedintermediatethatisstabilizedbytheoxyanionhole.Ans:tetrahetralSection:9.1Intrypsin,thespecificitypocketcontainsa/an______________residuethatbindstothepositivechargeoftheKorRresidueo