molecular biology lab：分子生物学实验室

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1、CELL3035,MOLECULARBIOLOGYLABSpring2011CatalogDescription:LaboratoryexperienceinmolecularbiologytechniquesPrerequisiteorCo-requisite:CELL311orCELL3110orCELL3030MolecularBiologyInstructor:NancyHopkinsOffice:SternHall,Room4009Phone:862-3162E-mail:nhopkin@tul

2、ane.eduOfficeHours:Monday10–11am;Tuesday2-3pm;Wednesday11am-noon;Friday1-2pmandothertimesbyappointment.Ifyoucannotcomeforofficehours,pleasearrangeanappointmentbye-mail.Textbook:LabPacketsforMolecularBiologyLab-CoursePacketavailableintheTulanebookstore.Ath

3、reeringbinder(1-2inches)isalsoneeded.ReadingswillberequiredfromAPhotographicAtlasfortheMolecularBiologyLaboratorybyPatrickGuilfoile.ThisbookisoutofprintandispostedonBlackboardforyouruse.Youmayfindinformationin“MolecularBiology”byRobertF.Weaverhelpful.This

4、isthetextforCELL3030.ThetextsforCELL3750and4010arealsousefulsourcesofadditionalbackgroundinformationfortheseexperiments.LearningOutcomes:·Practicebasiclaboratorytechniquesandsafety·UserestrictionenzymesandgelelectrophoresistoanalyzeDNA·Knowthebasictechniq

5、uesofblotting,probepreparationanddetectionmethods·BeabletoperformandanalyzeaPCR·BeabletomanipulateRNAinthelaboratoryincludingisolation,quantification,anddetectionofaspecificmRNAusingRT-PCR·Usemodernmolecularcloningtechniquestomodifyaplasmidtoexpressaprote

6、inanddetectthisexpression·BefamiliarwithmodernDNAsequencingmethodsandgenomedetermination·Beabletousereadilyavailablecomputermethodsforthemodernmolecularbiologylab·KnowhowtoworkcooperativelyinamolecularbiologylabClassmeetings:SternHall,Room3000M,T,W1-5pmPr

7、ogramOutcomes:1)Masteringandapplyingbroadknowledgeofbiologicalphenomenonandmechanisms.2)    Identifying,formulating,andsolvingbiologicalproblems.3)    Designingandconductingexperimentsatresearchorprofessionalsettings.4)    Collecting,analyzingandinterpret

8、ingdata.6)    Actingasacriticalteamplayer.7)Communicatingpreciselyandeffectively8)PossessingprofessionalandethicalresponsibilityProgramObjectives:Toprovideourstudentswithbroadknowledgeinthecellularandmolecularbasiso