荧光性双层膜的制备及其在汞离子检测中的应用毕业论文

《荧光性双层膜的制备及其在汞离子检测中的应用毕业论文》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、荧光性双层膜的制备及其在汞离子检测中的应用目录一前言5二实验部分62.1实验仪器与试剂62.1.1仪器62.1.2试剂62.2实验内容72.2.1自组装膜的制备72.2.1.1组装机理72.2.1.2Cys/Au的制备72.2.1.3HOX/Cys/Au自组装膜的制备72.2.28-羟基喹啉铝（8-羟基喹啉汞）的合成82.2.3Al(OX)3/Cys/Au的制备82.2.4自组装膜荧光光谱测定82.2.5电化学实验92.2.6膜的结构表征9三结果与讨论93.18-羟基喹啉和8-羟基喹啉铝溶液溶剂、激发波长和pH的选择93.1.1溶剂的选择93.1

2、.28-羟基喹啉和8-羟基喹啉铝溶液的激发波长的选择103.1.38-羟基喹啉和8-羟基喹啉铝溶液pH值的选择103.2自组装膜优化条件的选择113.2.1.1半胱胺酸在金表面上自组装的电化学表征113.2.1.2组装DL－半胱氨酸浓度的选择123.2.1.3组装DL－半胱氨酸时间的选择123.2.1.4组装DL－半胱氨酸pH值的选择133.2.2Cys/Au电极酸化时间和浓度的选择133.2.38－羟基喹琳荧光物质及其金属螯合物的组装133.2.3.1确定荧光物质的激发波长133.2.3.2浸泡时间与荧光物质浓度的选择143.2.3.3pH值的

3、选择143.3自组装双层膜的荧光光谱153.4自组装膜的表征163.4.1电极荧光光谱表征163.4.2膜的结构表征163.58－羟基喹啉铝、8－羟基喹啉溶液及自组装膜稳定性能的比较173.5.1溶液荧光强度的比较173.5.2稳定性的比较173.6Hg2+的荧光传感分析183.6.1汞离子的荧光传感的分析特性18四结论20五参考文献20AbstractSelf-assembledmonolayer(SAMs)canbedesignedaccordingtoourdemandinmolecularlevel，Ithasmanymeritswhic

4、hdon’texitinothermethods.NowSAMshasbeenextensiveappliedinelectroanalyticalchemistry.Butitisfewreportedaboutitsapplicationinfluorescenceanalysis.Fluorescenceanalysisisanimportantandeffectivespectrumanalysis，IfwecancombinecombineSAMstechnologywithfluorescenceanalyticalmethodand

5、developanotherinterfacefluorescenceanalyticalmethod，Itwaseffectiveindiminishingthesamplevolumewithaviewofon-chipanalysis，thereforehaspromisingprospectsinfluorescenceanalysisandchemicalsensor.Thefluorescenceintensityof8-hydroxyquinolineislow，inordertoimproveitsfluorescenceinte

6、nsity,inthispaperwesynthesizedfluorescencereagent8-hydroxyquinolinealuminum(Al(OX)3,Itisaverysignificantelectroluminscentmaterialandhasattractedgreatedattentionbothfromfundamentalandpotentialapplicationpointofview.Inthisexperiment,Theself-assembledbilayermembraneAl(OX)3/Cys/A

7、uwasfabricatedviaelectrostaticactionwithDL-cysteine(Cys)monolayersongold,surface.inordertocharacterizeditsstructure,wemakeuseofcyclicvoltammetry(CV)incombinationwithX-rayPhotoelectronspectra(XPS).Itwasfoundthattheself-assembledbilayermembraneAl(OX)3/Cys/Auhasgoodselectivityfo

8、rHg2+,soitcanbeappliedtothedetectionofmercuryion.Moreover,wefoundtha