针刺对脑缺血再灌注损伤大鼠外周血清sod、mda、mirna-126与vegf表达的影响

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1、万方数据北京协和医学院中国医学科学院硕士研究生学位论文EffectsofAcupunctureonPeripheralSerumExpressionofSOD，MDA，miRNA一126andVEGFinRatswithCerebralIschemiaReperfusionInjuryObjectiveAbstractThisstudywasbasedonthepreviousresearch，thecerebralischemiareperfusioninjury(CIRI)modelratsweremade

2、bysuture-occludedmethod．Toexploretheeffectsofacupunctureat“Baihui”(GV20)and“Zusanli”(ST36)ontheperipheralserumexpressionofSODandMDArelatedtooxidativestress，andontheexpressionofmiRNA一126andVEGFrelatedtovasculargenesisinratswithCIRI．MethodsHealthymaleSprague--

3、Dawleyratswererandomizedintoasham--operatedgroup(S)，amodelgroup(M)，anacupuncturegroup(A)，andanedaravonegroup(ED)usingarandomdigitstable．Eachgroupwasfurtherrandomlydividedintold，3d，5d，and7dfoursubgroupsbasedonthereperfusiontimesaccordingtoarandomdigitstable．I

4、nthegroup(M)andthegroup(A)，CIRlwasinducedusingthethreadocclusionmethod．ElectroacupuncturestimulationwasapplieddailytoGV20andleftST36for20min，attheindicatedtimepointsaftersuccessfuloperations．Edaravonewasgivenattheindicatedtimepointsaftersuccessfuloperationso

5、nceaday．TTCstainingwasusedfortestingmodel．SerumwassampledfordetectingVEGFproteinviaenzyme-linkedimmunosorbentassay，serumSODactivitywasexaminedusingXanthineoxidasemethod，serumMDAcontentwasevaluatedbyTBAmethod，andserummiRNA一126wasexaminedusingquantitativePCR．R

6、esults1．TTCstainingshowedthattherewasnon—infarctionchangeinthegroup(S)，whichbraintissuepresentedinredcolour，whileinthegroup(M)thecolourinthe6万方数据北京协和医学院中国医学科学院硕士研究生学位论文areaofinfarctioncorewasinwhitecolourcomparedwiththecommonredbraintissue．2．TheserumlevelofS

7、ODintheCIRIratsdecreasedsignificantly，andtheminimumexpressionofSODinboththegroup(M)andthegroup(A)occurredat3dExceptthetimepointofldingroup(A)，theexpressionofSODinthegroup(A)andthegroup(ED)washigherthaninthegroup(M)(P<0．05)，andthelevelofSODinthegroup(ED)wassi

8、gnificantlyhigherthaninthegroup(A)(P<0．05)．3．MDAexpressioninserumfromthecerebralischemiagroupwashigherthanthatintheingroup(S)．Comparedwiththegroup(M)，thelevelofMDAintheserumofthegroup(A)andthegr