软骨源性微载体复合大鼠骺软骨细胞修复大鼠骺板软骨缺损的实验分析

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1、解放军医学院硕士学位论文骨组织，其在第6周时．-j-以减轻骨桥组织的生成和减轻内翻畸形程度，但完全解决和修复骺板损伤造成成角畸形需要进一步的研究和探索。以软骨源性微载体为基础构建组织工化程骺板有可能成为一种骺板软骨缺损的修复材料。关键词：组织工程；软骨细胞外基质；微载体；生长板：骨桥2解放军医学院硕士学位论文ResearchonCartilageDerivedMicrocarriersCompoundedRats’ChondrocytestoRapierRatEpiphysealPlateCartilageDefectsAbstractObjects：Researchaim

2、atbuildingalarge-sizeratepiphysealplatedefectmodelandapplyingthismodeltoevaluaterepairofratepiphysealplatedefectbyusingcompositeofcartilagederivedmicrocarriersandrats’chondrocytes．Methods：Usethelocatortoinserttheratepiphysealcartilagecentralontherightproximaltibial，carefullyscrapethemedica

3、lsemi-epiphysealcartilageandfinallybuildalarge—sizeratepiphysealplatedefectmodel．Xrayassay,Micro-CTevaluation，histologicalevaluationsareusedtoevaluatevarusdeformityformationandbonebridgetissueformation．PCRisusedtoevaluatethemechanismofbonebridgetissueformation．Cartilagederivedmicrocarriera

4、samajormaterialisusedtocombinewithalginatehydrogelstoformepiphysealplateshapehydrogelscaffold．Thescaffoldiscompoundedwithratepiphysealplatechondrocytestorepairratepiphysealplatedefect．Severalcontrolgroupsaresetupforcomparison．Usinggrossmorphologyevaluation，Xray,histologicalstainingtoevalua

5、tetheefficiencyofrepair．Results：Aftermedialproximaltibialepiphysealplateinjury,allratsappearvarusdeformityandcan’tberectifiedbythemselves．TheLocatorcaneffectivelyprotecttheouterepiphysealplatefromdamage．After1-weekinjuIMbonebridgetissuesoccurandVail,ISdeformitywerebeenfound．Bonebridgetissu

6、ehavegeneratedwithintheprocessinvolvedintheformationofbonecartilage．Cartilagederivedmicrocarrierscombinedwithalginatehydrogelsandthenratepiph)7sealchondrocyteswereseededonthescaffoldtorepairdefect．ThecombinationCaneffectivelyformalargenumberofnative—likeepiphysealcartilageindefectarea．Afte

7、r6weeks，thecombinationcanpreventvarusdeformityofbonetissueformationandreducethebonebridge(P<0．05)．Howeverin12weeks．internalvarusdeformitycorrectionarenotobvious(P>O．05)．Controlgroupsshownoorliulenewborncartilage—liketissueformation．Conclusion：TheLocatorandscra