urocortin+i预处理抗心肌缺血再灌注损伤的线粒体机制分析

《urocortin+i预处理抗心肌缺血再灌注损伤的线粒体机制分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、遵义医学院硕士学位论文顾燕优于I/R组和5-HD+UcnⅠ组（P＜0.05）；5-HD+UcnⅠ组与I/R组比较3态呼吸、呼吸控制率、烟酰胺腺嘌呤二核苷酸氧化酶、琥珀酸氧化酶差异有统计学意义（P＜0.05或P＜0.01），而细胞色素C氧化酶活性无统计学意义（P＞0.05）；3.线粒体高膜电位比例的变化：Nor组心肌细胞线粒体高膜电位比例较其余组高（P＜0.01），而I/R组和5-HD+UcnⅠ组低于UcnⅠ组（P＜0.05），I/R组与5-HD+UcnⅠ组比较差异无统计学意义（P＞0.05）；4.心肌ATP含量的变化：

2、Nor组心肌细胞ATP含量较其余组高（P＜0.01）；UcnⅠ组心肌细胞ATP含量高于I/R组和5-HD+UcnⅠ组（P＜0.05）；5-HD+UcnⅠ组与I/R组比差异有统计学意义（P＜0.05）。结论1.UcnⅠ预处理可增加缺血前正常鼠心心肌收缩力、降低舒张末压，并改善缺血再灌注后心脏功能；2.UcnⅠ预处理主要通过开放mito-KATP通道，改善线粒体呼吸功能及呼吸酶活性，保护线粒体结构和功能的完整，稳定线粒体膜电位，维持呼吸链电子的传递及氧化磷酸化的正常进行，保存心肌细胞内ATP含量，保证再灌注时受损心肌复苏的

3、能量供给。关键词：心肌缺血再灌注损伤；UrocortinⅠ；预处理；线粒体；心肌保护；线粒体敏感性钾通道。3万方数据遵义医学院硕士学位论文顾燕ResearchofmitochondrialmechanismonUrocortinIPretreatmentofmyocardialischemia–reperfusioninjuryAbstractObjective::ToinvestigatethemitochondrialmechanismofcardioprotectiveeffectofUrocortinIpret

4、reatmentonmyocardialcell.Methods:RatmodelofischemicreperfusioninjurywaspreparedusingtheLangendorffisolatedheartperfusionsystem.HealthySD48maleratswererandomlydividedintofourgroups:normalgroup(Norgroup),Ischemiagroup(I/Rgroup),UrocortinⅠpretreatmentgroup(UcnⅠgrou

5、p),5-HDantagonismgroup(5-HD+UcnⅠgroup).Norgroup:perfuseKerbs-Henseleitbuffercontinuouslyfor155minutes.I/Rgroup:perfuseST.Thomascardioplegicsolutionatthetemperatureof4℃beforeglobalcardiacischemiafor40minutesatthetemperatureof32℃,thenreperfusefor60minutes.UcnⅠgrou

6、p:perfuseK-Hbufferfor30minutesbeforeischemia,therestmanagementsaresamewithI/Rgroup.5-HD+UcnⅠgroup:perfuseK-Hbuffercontaining5-HDbeforeUcnⅠpretreatment,therestmanagementsaresamewithUcnⅠgroup.Thefollowingindexesofthefourgroupsareevaluatedattheendofequilibrium,befo

7、reischemia,attheendofreperfusion,respectively.(1)Indexesofheartfunction:heartrate(HR),leftventricularend-diastolicpressure(LVDEP),leftventriculardevelopedpressure(LVDP)andmaximumdp/dt(dp/dtmax).(2)Extractmitochondriafromventriculartissues,analyzerespiratoryfunct

8、ionandrespiratoryenzymeactivityusingoxygenelectrode.(3)Microstructuresofmyocardialcellwerescannedbytransmissionelectronmicroscope.2.Isolatemyocardialcellsofadultratus