五味子提取物对小鼠辐射损伤的免疫保护作用与其机制分析

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1、英文摘要countinginstrument．TheabsolutenumberofCD3十，CD4十andCD8+Tcellsinmice’Speripheralbloodafter60Co．丫irradiationwasmeasuredbyflowcytometry．ContentIgGandC3inmice’serumwereanalyzedbyimmunologicalturbidimetry．3CellmophalolyofthymustissueinmicewasobservedbymicroscopeafterGimsast

2、aining．Cellapoptosisofthymusinmicewasanalizedbyflowcytometry．4Expressionofbcl-2andFasmRNAinthymustisseofmicewasdetectedbyRT-PCR．5Expressionofbcl一2andFasproteininthymusofmicewasdetectedbyimmunohistochemistry．Results：1Afterirradiation．thenumberofWBCandLYMinperipheralbloodof

3、NStreatedmicewaslowerthanthatofnormalcontrolgroup(尸O．05)．2Afterirradiation，theabsolutenumberofTlymphocytesubsetsinmice’peripher

4、albloodwasdecreasedinNStreatedmicethanofnormalsodiumtreatedgroup(尸

5、upandnormalsodiumgroup(P>O．05)．3Afterirradiation．thenumberofthymuscellwas5英文摘要increased，cellapoptosisrateofthymusinSCtreatedmicewaslowerthanthatofNStreatedgroup(cellapoptosisratewas2．87士1．03％and21．32+2．56％respectively,尸

6、ndnormalsodiumgroup俨>O．05)．4Afterirradiation，expressionofbcl-2mRNAinthymustissueofNStreatedmicewasdownregulated，andexpressionofFasmRNAwasupregulatedafterirradiation．UndergoneSCtreatment，expressionofbcl一2mRNAinthymustissuewashigherandFasmRNAwaslowerthanthatofinNSgroup(P<0．

7、01)．5Afterirradiation，expressionofbcl一2proteininthymustissueNStreatedmicewasupregulated，andFaswasdownregulated．UndergoneSCtreatment，expressionofbcl-2inthymustissuewashigherandFaswaslowerthanthatofinNSgroup(P

8、tioneffectonirradiationinmice．2Theextractofschisandrachinensiscouldpreventtheapoptosisofthymusce