缺血后处理对糖尿病大鼠局灶性脑缺血再灌注损伤线粒体功能的影响

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1、山东大学硕士学位论文(1)I-Post可减轻缺血组织的再灌注损伤现象同样存在于脑组织；(2)I-Post对近期神经行为学的改善作用不明显；(3)I-Post可以抑制糖尿病大鼠脑神经细胞凋亡，减轻糖尿病大鼠局灶性脑缺血再灌注的病理损伤；(4)I-Post可以抑制糖尿病大鼠缺血脑组织线粒体超微结构的改变，提高线粒体ATPase活性，增强线粒体清除氧自由基的能力，减少脂质过氧化物的生成，从而起到线粒体保护，减轻缺血再灌注损伤的作用。[关键词]缺血后处理；脑缺血：再灌注损伤；糖尿病，实验性；细胞凋亡：线粒体山东大学硕士学位论文Roleo

2、fmitochondriainischemicpostconditioning—inducedattenuationofcerebralischemiaindiabeticratsObjectiveSpeciality：NeurologyPostgraduate：LuYingyunSupervisor：Prof．WangCuilanABSTRACTToestablishanmodeloffocalcerebralischemia-reperfusioninstreptozotocin—induceddiabeticrats；To

3、exploretheeffectofischemicpostconditioning(I-Post)oncorticalneuronsapoptosisinfocalcerebralischemiaofdiabeticrats；ToinvestigatetheeffectofI-Postonthestructureandfunctionofmitochondriaafterfocalcerebralischemia··reperfusioninstreptozotocin--induceddiabeticrats；TOdebat

4、ethemechanismofI-Postinacutecerebralischemiacomplicated、析thdiabetes．Methods(1)ThemodelsofexperimentaldiabetesratswereestablishedbyasingleintraperitonealinjectionofSTZ(60mg／kgbodyweight)，andthesuccessfulmodelswereselectedtoestablishthemodelsoffoealcerebralischemia-rep

5、erfusionratsbyLonga’Stechnique．Sixty—fiveratsweresuccessfullymadeintodiabeticmodelsandrandomlydividedinto4groups：1)controlgroup：诵thnootherintervention；2)Sham-operatedgroup：thesurgicalprocedurewasidenticaltoothergroups，buttheMCAwasnotligated；3)I／Rgroup：thebrainWasunde

6、rgoneischemiafor90minfollowedbyreperfusionfor6h；4)I-Postgroup：ratsweresubjectedto3cyclesof15s／15sreperfusion／reocclusionafter90minofischemia．(2)Postconditioningprotocols：thebrainwassubjectedtomiddlecerebralarteryocclusionfor90min，thentreatedfor15see，15sec，15secbybrie

7、f4山东大学硕士学位论文reperfusionconsecutively，eachwasseparatedwithocclusionfor15sec，followedbyapersistentreperfusion，whichmadethetotalreperfusiontimethesameastheotherthreegroups．(3)TheassessmentstandardofLonga‘5scoresWasappliedtoassesstheneuralfunctionoftheratsaftercerebralis

8、chemia-reperfusion．(4)TTCstainingWasappliedtoobserveandnotetheformationofinfarctionzone；Thepathologiccharacteristicsofcerebralische