脑缺血大鼠窦房结的形态结构与心室肌cx43表达的变化

《脑缺血大鼠窦房结的形态结构与心室肌cx43表达的变化》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、中文摘要结论1局灶性脑缺血后未发现大鼠心脏窦房结区结构出现病理改变。2大鼠局灶性脑缺血导致其心室肌连接蛋白Cx43表达减少和空间分布异常，引起心肌间电信号传导障碍。关键词：脑缺血；Cx43；窦房结；心电图英文摘要ChangesofSinoatrialNodeandVentricularConexin43ofCerebraIlschemiaRatsCandidateformaster：GuoYiweil’2Supervisor：ProfessorZangWeidon91ProfessorWenXiaojn2，Wangxing

2、1．Departmentofanatomy，themedicalcollegeofZhengzhouUniversity，Zhengzhou,Henan4500522．DepartmentofAnatomy，XinxiangMedicalUniversity，Xinxiang，Henan453003AbstractObjectiveToinvestigatethemorphologicalchangeofsinoatrialnode(SAN)andtheexpressionofconnexion43(Cx43)iIllef

3、tventricleofcerebralischemiaratfromproteinandRNAlevel．Providereliabletheoryanddataforrecognition,preventionandtreatmentofcerebral-cardiacsyndrome(ccs)．MethodsAtotalof72healthmaleSprague—Dawleyrats，weight2509'一3509，weredividedi11tocontrolgroup(11=12)andMCAOmodelgro

4、up(MCAO，n=60)，MCAOgroupweresubdividedinto3k24tl，48k72ll，and1weekgroup．AccordingtothemodifiedZeaLongapatchingmethod，performleftmiddlecerebralarteryocclusion．TheSUCCESSofratmodelidentifiedbytherightlimbmovementdisorder2hoursaftersurgery．Controlgroupweretreated、析tllt

5、hesameprocedurewithouttheleftmiddlecerebralarteryocclusion．①MeasurementofECG．②TTCstainingofdamagedbrainareasafterMCAO．③HEstainingandtransmissionelectronmicroscope，TEM：SANtissuewerecutfromsulcusterminals(frontrientsectionofthesuperiorvenacavaproximalwall)andperform

6、edHEstainingandelectronmicroscopy．④Immunofluoreseentstaining，Cx43：somefrozensectionsofIV英文摘要leftventriclewerestainedandobservethechangesofCx43．⑤RT．PCR：detecttheexpressionofCx43mRNAineachgroup．⑥Statisticalanalysis：immunofluorescentresultswereanalyzedbyImageJ1．44e，S

7、tatisticalanalysiswasperformedusinganalysisofvarianceandexpressedasmean+SD．P<0．05Wasconsideredstatisticallysignificant．ReSUl缸lECGanalysisDemonstratedthatincontrolgroup，heartratechangesweresignificantlyincreasedaftersurgery伊<0．05)，butafter3hheartrategraduallyreturn

8、edtothepreoperativelevel．Experimentalratsaftermiddlecerebralarteryocclusionshowedsignificantlyincreasedheartrate，he眦rateacceleratingWasmostobviousafter3