见血清质量标准研究

《见血清质量标准研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、中图分类号：R28硕士学位论文见血清质量标准研究院（系）、所药学院研究生姓名侯杰荣学科、专业中药学导师姓名税丕先教授二Ο一二年四月目录1见血清质量标准研究„„„„„„„„„„„„„„„„11.1中文摘要„„„„„„„„„„„„„„„„„„„„„11.2英文摘要„„„„„„„„„„„„„„„„„„„„„31.3前言„„„„„„„„„„„„„„„„„„„„„„„51.4仪器和试剂„„„„„„„„„„„„„„„„„„„„71.5方法和结果„„„„„„„„„„„„„„„„„„„„81.6讨论和结论„„„„„„„„„„„„„„„„„„„„251.7参考文献„„„„„„„„„„„„„

2、„„„„„„„„301.8英汉缩略词对照表„„„„„„„„„„„„„„„„„312致谢„„„„„„„„„„„„„„„„„„„„„„„323综述„„„„„„„„„„„„„„„„„„„„„„„334在读期间发表的论文复印件（不含首页、目录）见血清质量标准研究摘要目的：鉴于目前见血清药材尚无规范的质量标准，仅见收载其来源、性状、功效，无根、茎、叶、果实、种子及粉末等的显微特征的鉴定标准，无总灰分及酸不溶性灰分的测定标准，无浸出物和水分等的测定标准。因此，见血清的质量标准有待于进一步的提高和完善。本研究旨在通过对见血清的质量标准展开研究，制定其质量标准的草案，对进一步开发和利用见

3、血清资源提供科学依据。方法：1.对见血清的植物形态和性状进行生药学的鉴定；2.对见血清的根、茎、叶、果实切片进行显微观察；3.对见血清粉末制片进行显微观察；4.对见血清的成分进行提取分离，进行初步的理化分析，为进一步确定其有效成分提供参考；5.按照中国药典2010版一部附录所载方法测定见血清药材的总灰分、酸不溶性灰分、水分含量、水溶性浸出物含量；6.用1,3-二亚油酸甘油酯为对照品对其进行薄层色谱试验，作为见血清质量标准鉴别的方法之一；7.采用酸碱两相滴定法测定见血清的总生物碱的含量。结果：通过查阅相关文献和进行合理的实验获得了相应的技术指标和数据：1.对见血清的原植物形

4、态和根、茎、叶、果实、种子的显微形态进行描述：详见论文相应部分；2.见血清全草水溶性浸出物热浸法测定含量在20.97%～21.86%，平均含量在21.45%，故暂定其水溶性浸出物热浸法不低于20.0%；3.见血清含水量在12.80%～13.20%，平均值为12.92%，暂定其水分含量不高于13.0%；4.见血清总灰分幅动范围在8.03%～8.78%，平均值为8.43%，故暂定为其总灰分不高于9.0%；5.见血清酸不溶性灰分幅动范围在2.23%～2.57%，平均值为2.40%，故暂定见血清酸不溶性灰分不高于3.0%；6.通过酸碱滴定法，测得见血清总生物碱的含量范围在0.04

5、22%～0.0512%，故暂定见血清总生物碱含量不低于0.04%；7.薄层色谱试验发现见血清与1,3-二亚油酸甘油酯具有相同的斑点。结论：本实验通过对见血清质量标准的研究，完善和提高了见血清的性状鉴别、理化鉴别、显微鉴别等，其中新增了见血清的粉末鉴别、组织切片观察、总灰分、酸不溶性灰分、水分、浸出物等的鉴别标准，同时通过酸碱两相滴定法测定了见血清总生物碱的含量，形成了见血清质量标准的草案，为见血清的进一步开发和利用提供了科学依据。关键词：见血清；质量标准；总生物碱;1,3-二亚油酸甘油酯ThequalitystandardsresearchofLiparisnervosa

6、HerbaAbstract:Objective:BecausethereisnonormativequalitystandardofLiparis-nervosaHerba,onlyposteditsorigin,character,efficacy.Noroots,stems,leaves,fruits,seedsandpowdercharacteristicsoftheevaluationstandard,nototalashandacidinsolubleashcontentinspecttion,noextractandwatercontentoftheexami

7、n-ation.So,Liparis-nervosaHerba´squalitystandardstobefurtherimprovedandperfected．ThisstudyofLiparisnervosathroughresearchonpharmacognosyandqualitystandards，establishthedraftofthepharmacognosyandqualitystandards,forfurtherdevelopmentandutilizationofLiparisnervosareso