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ID:32855003
大小:3.31 MB
页数:56页
时间:2019-02-16
《钙、钾营养对nacl胁迫下马铃薯生长和生理特性的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、分类号S532学校代码10129UDC633学号09202029钙、钾营养对NaCl胁迫下马铃薯生长和生理特性的影响EffectsofCalcium,PotassiumnutritiononGrowthandPhysiologicalCharacteristicsofPotatounderNaClStress申请人:王朝霞学科门类:农学学科专业:作物栽培学与耕作学研究方向:马铃薯栽培生理与品种改良指导教师:蒙美莲教授论文提交日期:二〇一二年六月摘要以马铃薯克新一号品种为试验材料,采用组织培养的方法,研究了不同NaCl胁迫浓度下向培养基中分别添加
2、不同浓度的CaCl2和K2SO4对马铃薯脱毒苗生长和生理指标的影响。主要研究结果如下:1培养基中添加5~15mmol/LCaCl2均可不同程度增加各NaCl胁迫浓度下马铃薯脱毒苗株高、茎粗、叶片数及茎叶干重,还能有效提高25mmol/L和50mmol/LNaCl胁迫浓度下马铃薯脱毒苗的根系活力。2低盐胁迫浓度下,5~15mmol/LCaCl2均不同程度的促进了马铃薯脱毒苗脯氨酸、可溶性糖和可溶性蛋白含量的增加。相同胁迫浓度下,SOD和POD活性均随CaCl2浓度的增加呈先增高后降低趋势,且CaCl2浓度为10mmol/L时,SOD和POD2+活
3、性最大。另外,Ca的添加还有效降低了叶片细胞膜透性和MDA含量。高盐胁迫浓度下,5mmol/LCaCl2处理的马铃薯脱毒苗生长较好。3低盐胁迫浓度下,4~12mmol/LK2SO4不同程度的促进了马铃薯脱毒苗株高、茎叶干重的增加及根系活力的增大,且8mmol/LK2SO4处理好于其它浓度。高盐胁迫浓度下,4mmol/LK2SO4处理的马铃薯脱毒苗茎叶生长最好。4各NaCl胁迫浓度下,培养基中添加4~8mmol/LK2SO4均不同程度的促进了+马铃薯脱毒苗脯氨酸和K含量的增高,SOD和POD活性的增大。适宜浓度的K2SO4+还有效降低了叶片MDA
4、和Na含量,且低盐胁迫浓度下,8mmol/LK2SO4处理效果优于其它浓度;高盐胁迫浓度下,以4mmol/LK2SO4处理效果最好。关键词:马铃薯;NaCl胁迫;钙、钾营养;生长发育;生理特性EffectsofCalcium,PotassiumnutritiononGrowthandPhysiologicalCharacteristicsofPotatounderNaClStressAbstractTheeffectsofdifferentcalcium,potassiumnutritionconcentrationongrowthdevelo
5、pmentandphysiologicalcharacteristicofpotatovariety“gramnewNo1”wereinvestigatedunderNaClstressundertissuecultureconditions.themainconclusionswereasfollows:1UnderdifferentNaClconcentrationstress,5~15mmol/LCaCl2treatmentcouldincreaseplantheight,stemdiameter,leafnumberandthedryw
6、eightofstemandleafofpotatoandcouldimprovedtherootactivityofpotatounder25mmol/Land50mmol/LNaClstress.2Underthelow-saltstress,5~15mmol/LCaCl2treatmentpromotedtheincreasingofprolinecontent,solublesugarcontent,solubleproteincontent.Underthesamesaltconcentrationstress,superoxided
7、ismutase(SOD)activityandperoxidase(POD)activitywasincreasedfirstlyandthendecreasedwiththeincreasingofCaCl2concentration.AndwhenCaCl2concentrationwas10mmol/L,superoxidedismutase(SOD)activityand2+peroxidase(POD)activitywerethelargest.Caalsopromotedthedecreasingofcellmembranepe
8、rmeabilityandMDAcontenteffectively.Butunderthehigh-saltstress,theeffectof5~
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