肺炎克雷伯氏菌的蛋白质组学分析

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1、硕士学位论文肺炎克雷伯氏菌的蛋白质组学分析院（系）、所临床医学院研究生姓名曾白华学科、专业临床检验诊断学导师姓名王开正教授二Ο一二年三月目录摘要.....................................................1前言.....................................................6材料与方法...................................................91实验材料...................................

2、................91.1标准菌株................................................91.2实验菌株................................................91.3主要试剂来源............................................91.4主要试剂配制...........................................101.5主要实验器材.................................

3、..........101.6药敏纸片...............................................112实验方法..................................................112.1ESBLs仪器初筛实验.....................................112.2产ESBLs菌株表型确证实验..............................112.3细菌保存.............................................

4、.122.4细菌复苏..............................................122.5细菌蛋白提取..........................................132.6细菌蛋白保存..........................................132.7制备样品混合液........................................132.8芯片活化..............................................132.9上样...

5、...............................................142.10质量控制.............................................143质谱检测..................................................143.1检测标准化............................................143.2质谱仪检测样本........................................1424数据采集......

6、............................................165数据分析..................................................165.1ESBLs菌株的蛋白质决策树诊断模型的建立.................165.2细菌蛋白质组学同源性分析..............................17结果....................................................181产ESBLs表型确证试验.................

7、.....................182肺炎克雷伯氏菌蛋白质指纹图................................193肺炎克雷伯氏菌产ESBLs菌蛋白决策树诊断模型................214聚类分析..................................................244.1聚类方法..............................................244.232株产ESBLs细菌聚类分析结果..........................264.349株

8、肺炎克雷伯氏菌聚类分析结果........................27讨论......