下丘脑后部gaba对大鼠睡眠-觉醒周期的影响及其作用机制研究

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1、Corporation,Tokyo，Japan)，signalsweredigitizedbYCEDMicro1401MKⅡ(CambridgeElectronicDesignLimited，London,切目andcomputerrunningtheSpike2(CED)recordingsystem．A24hbasicwake-sleepstatesincontrolgroupwererecordedandanalyzedbymanu2Lllyscoredin30SepochsonthedigitizedEEG／E

2、MGOnthe8thday,afree#assmicropipette(10-20111111tipdiamete0containingibotenicacidsolutionforspecificcelldamage(10nM／0．5Il1，Sigma)WaSinjeetedintotheVLPOoneachsideafterbonewaxremovedunderanesthetization．Oneweekafterthesecondsurgery,ratsofVLPOlesiongroupwererecorded

3、sleep—wakefulnessstatesfortwoweeks．Onthe10thdayafterlesionofVLPO，bicuculline(10nM／0．5la1)，aGABAA-receptorantagonist,wasmicroinjectedintotheTMnandrecordedsleep-wakefulnessstatesfor24h．RESULTS：(1)Theratioduring24hofW,SWSlwererespectivelyincreasedby13．17％俨

4、．9％p

5、4hsleep-wakestatesshowedthattheratioofW,SWS1WererespectivelyincreaSedby12．61％(尸

6、esionsuggeststhatGABAergicneuronsintheVLPOplay811importantroleinmaintainingsleep．Thedataofbicuculline，aGABAA-receptorantagonist，microinjectionintoTMnafterVLPOlesionelicitedwakefulnessandsleepdepressionfor50In也bycontrariesincreasedsignificantlylightslowwavesleepf

7、or24hsuggestthattheGABAergicneuronsintheTMnrelatetosleepdrive(sleepiness)vialocalcircuittoinhibitdirectlyhistaminergicneurons．KeyWords：OABAergicneurons，GABAA-receptorantagonist,histaminergicneuron,neurotoxiclesion,tuberomammillarynucles，ventrolateralpreopticarea

8、英文缩写GABAPSPSGSWSSWSlSWS2VLPOW英文缩略词英文名中文名Gamma-aminobutyricacid7-氨基丁酸组织胺Paradoxicalsleep异相睡眠Polysomnography多导睡眠描记Slowwavesleep慢波睡眠Lowslowwavesleep浅慢波睡眠Deepslowwaveslee