几种国兰组织培养与春兰菌根真菌-研究

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1、ABSTRACTABSTRACTChineseorchidsgenerallyrefertopartoftheterrestrialorchidsofCymbidium，suchasC．goeringii，C．faberi，C．ensifolium，C．sinense，C．kanran，withhighornamentalvalueandeconomicvalue．Chinaisrichinnativespeciesandcultivarsresources．Mycorrhizalfungiestablishmutuallybeneficialsymb

2、iosisrelationshipwithorchidsundernaturalconditions，andplayacrucialroleincompletingorchidslifecycle．Thepurposeofthisstudyistoanalyzethefactorsinfluencingonorchidtissueculture，andestablishsymbioticsystemwithorchidsandmycorrhizalfungibyartificialmoculmiontoexploreitssymbioticeffect

3、s．Themainachievementsareasfollows：1．ThebestmediumforrhizomemultiplicationandbuddifferentiationofCkanranwasMSwith6-BA1．5mg·L～andNAA0．3mg·L‘1；thebestmedium．forrhizomemultiplicationandbuddifferentiationofC．faberiwasMSwith6-BA1．0mg·L。1andNAA0．3mg。L‘1；duringtheC．goeringiitissuecultur

4、eprocess，it'sfoundthat1／2MSasthebasicmediumwith6-BA0．1mg。L’。，NAA5．0mg·L～andACl．OgL。1isbestfortherhizomemultiplication；with6-BA1．0mg·L～andNAA0．3mg·L。1isbestfortherhizomebuddifferentiation；withIBA1．0mg。L—andAC1．09L。1ismostconducivetoitsseedlingrooting．2．TissueculturesofCgoeringiiw

5、ereinducedonculturemediumwiththefungalelicitor．MycorrhizalfungalelicitorsisolatedfromtherootofwildCgoeringiiplayasignificantroleonpromotingrhizomemultiplicationandbuddifferentiation．3．PlantletsinvitroofCgoeringiiwereCO-culturedwith12fungalstrainsbyartificialinoculation．Afterinoc

6、ulatedwithstrainsFC1fortwomonths，thebiomassoftheseedlingsincreasedsignificantly,aswellascarbohydrate，waterandnutrientelementscontents．，whilepromotingthedifferentiationofseedlingroot．Byobservingparaffinsectionsandre。separmionresults，itseemsthatthestraincanformedasymbioticsystemwi

7、thCgoeringiiplantlets．4．DifferentvarietiesofCgoeringiitissue-cultureseedlingswereinoculatedwithmycorrhizalfungi．Afterinoculatedfortwomonths，theresultsshowthatunderthesameIIABSTRACTfungalstrains，‘yeyi’cangrewbetterwithmycorrhizalfungi，but‘huanqiuheding’grewweakerwithmycorrhizalfu

8、ngi．5．ByobservationthemycorrhizalstructureofC．g