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1、南京农业大学学报 2004,27(1):11～14JournalofNanjingAgriculturalUniversity光照强度和CO2浓度间接调控对甘薯无糖组培苗光合特性的影响12134徐志刚,崔瑾,焦学磊,丁为民,李式军(11南京农业大学农学院,21生命科学学院,31工学院,41园艺学院,江苏南京210095)摘要:以自行研制的组培苗光合速率测量系统检测了叶用甘薯无糖组培苗的光合速率,定量分析了外环境中CO2浓度和光合光量子通量密度(PPFD)对无糖组培苗光合特性的影响。结果表明:采用透气封口材料和自然换气,间接合理调控PPF
2、D和外环境CO2浓度对组培苗净光合作用产生积极促进作用;仅提高PPFD或外环境CO2浓度不能有效促进光合作-2-1用。当使用透气率为014的封口材料时,甘薯无糖组培苗的光合作用在光合光量子通量密度250μmol·m·s和外环境-1CO2浓度8735μmol·mol时最为适宜。关键词:组培苗;光合光量子通量密度;CO2浓度;光合特性中图分类号:Q945111;S123文献标识码:A文章编号:10002030(2004)01001104EffectsofphotosyntheticphotonfluxdensityandCO2concent
3、rationonphotosynthesisofsugar2freemicropropagationofsweetpotatoplantletsXUZhi2gang,CUIJin,JIAOXue2lei,DINGWei2min,LIShi2jun(11CollegeofAgriculture,21CollegeofLifeSciences,31CollegeofEngineering,41CollegeofHorticulture,NanjinAgriculturalUniversity,Nanjing210095,China)Abst
4、ract:Thenetphotosyntheticrateofsugar2freemicropropagationofsweetpotato(IpomoeabatatasLamk)plantletswasobservedbytheauto2measuresystemdesignedbyourselves.Theeffectsofphotosyntheticphoton2quantafluxdensity(PPFD)andCO2concentrationout2sidevesselonphotosyntheticcharacteristi
5、csofsweetpotatoplantletswereanalyzed.Theresultsindicatedthatwhenthegaspermeablemi2croporousfilmwasadoptedfoenatureventilationandthePPFDandCO2concentrationoutsidevesselwerewellcontrolled,thephotosynthe2sisofplantletsinvitrowouldbepromotedeffectively.Itwasinefficienttoprom
6、otethephotosynthesisofplantletsonlyenhancingeitherPPFDorCO2concentrationoutsidevessel.ThenetphotosyntheticrateoftheplantletswassignificantlyenhancedwhenthePPFDandCO2-2-1-1concentrationoutsidethevesselandthenumberofexchangesofthevesselwere250μmol·m·sand8735μmol·moland014,
7、re2spectively.Keywords:planttissueculture;photosyntheticphoton2quantafluxdensity;CO2concentration;photosyntheticcharacteristics密闭的组培容器满足了组培苗生长所需的无菌要求,却造成了不良的组培微环境,如:高相对湿[1]度,低光合光量子通量密度(PPFD),极低CO2浓度(光照期)和高CO2浓度(黑暗期)等。低PPFD[2,3]和低CO2浓度制约组培苗光合性能,迫使其转作异养或兼养生长,为此必须在培养基内添加外源糖
8、[4]以提供生长所需的碳源,而糖又会产生光合抑制效应。可见,组培苗的生长方式受到微环境的强烈影响。无菌组培环境和气密性小容器使组培苗光合作用的研究受到制约,也使得采用强制通气方法对组培[5]微环境的直接调控
