ph胁迫对三种养殖对虾生理生化指标的影响

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1、●■上海海洋大学硕士学位论文StudyonEffectsofpHstressonPhysiologicalandBiochemicalindexesinthreespeciesofshrimpsABSTRACTWaterisshrimplivingenvironment，SOwaterqualityhaveadirectimpactonthegrowthandeconomicbenefitsofshrimp．InammoniaN，pH，dissolvedoxygen，temperatureandsalinit

2、yphysiochemicalindexs，pHisoneofimportantphyrsiochemicalindexandoneofsynthesisreactionindexofwaterchemical’traits，canaffectwaterbiologicalproductivity．Atpresent，thestudyaboutpHstressonshrimpgrowth，iontransportenzymeandimmunerelatedindexshavereportedinthedome

3、sticandforeign．However，thecomprasionstudyofpHstressinFenneropenaeuschinensis，LitopenaeUSvannamei，Marsupenaeusjaponicushavn’tseenuntilnow．thisthesisselectedpHastheexperimentalchangefactorandstudiedcomprasionofpHstressongrowth，survivalrateandactivityofion—tra

4、nsportenzyme，immunerelatedfactorsinFchinensis．L．vannamei，M．japonicas．Thisstudyincludethreeparts。Thefirstpart：Thesurvivalrate，舀llNr。K，．ATPaseactivities，serumiNOS．PO。SOD，GSH-PX，LSZactivitiesandmuscleRNA／DNAwerecomparedinFchine．nsis．,LvannameiandMjaponicuswhic

5、hwererearedundercontrolgroup(pn8．2)，low(pn7．2)，andhi曲(pH9．2)pHstressconditionsinthisstudy．Resultsshowedthattherewerenonegativeeffectamongthreespeciesofshrimpswhenrearedinthecontrolgroupfor96h．UnderloworhighpHstressconditions，thesurvivalratesofMjaponicus,L．v

6、annameiandEchinensisfor96hwere96．7％and97．O％。60．1％and54．6％，58．6％and13．3％，respectively．、研threspecttogillNa+-K+-ATPaseactivity,三．vannameishowedhighestlevelandMjaponicuswaslowestundercontrolgroup．Thechangeon百llNd-K十一ATPaseactivityofEchinensisWasthebiggest．w11il

7、ethatofM．japonicaswassmallestunderbothlowandhighpHstressconditions．Also，allthreespeciesofshrimpstendtochangemuchmoreingillNa+．K+一ATPaseactivityunderpH9．2．Inthecontrol，theactivitiesofnonspecificimmunityrelatedenzymesofbothL．vannameiandM．japonicusweresignific

8、antlyhigherthanthatofF-chinensisandPO．Ⅳ●上海海洋人学硕士学位论文andSODactivitiesofM．japonicusweremarkedlyhigherthanthatofL．Pannamei．whiletheLSZactivityofL．vannameiwashigherthanthatofMjaponicus．Thechangesinnonspeci