mir29a在冠心病慢性心力衰竭患者外周血内的表达和意义

《mir29a在冠心病慢性心力衰竭患者外周血内的表达和意义》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、Abstract腹肘静脉血5ml，EDTA-2K抗凝，用1.5mlEP管进行分装，每管200ul，分装后置于-80℃保存。（2）通过生物信息学软件确定课题研究的miRNA为miR-29a，并针对miR-29a设计茎环反转录引物和Q-PCR引物。（3）用TRIzolLS分别提取冠心病慢性心力衰竭患者和健康对照个体血液样本的总RNA，进行反转录和Q-PCR。（4）用统计分析学软件SPSS17.0对冠心病慢性心力衰竭患者和健康对照个体的Q-PCR结果进行统计分析。结果（1）通过生物信息学软件预测出PPARδ可能是miR-29a的靶基因。（2）通过t检验，结果显示健康对照组与病例组外周血内miR-

2、29a的水平差异具有统计学差异。（3）通过单因素方差分析，结果显示健康对照组与各级病例组外周血内miR-29a的水平差异具有统计学差异。2（4）通过t检验和X检验，结果显示健康对照组和NYHA各级病例组各组间性别、年龄差异不具有统计学差异。（5）通过多元logistic回归分析，结果显示对照组和分级病例组各组内不同年龄、性别患者外周血内miR-29a的表达差异不具有统计学意义。（6）通过相关性分析，结果显示各组外周血内miR-29a水平与NT-proBNP水平显著正相关。结论与健康对照组相比，在冠心病慢性心力衰竭患者外周循环血内miR-29a表达上升；心衰等级越高，表达上升的倍数越高；外周

3、循环血内miR-29a的水平与年龄、性别无关，与心衰等级相关。关键词：冠心病慢性心力衰竭外周血miR-29a生物标志物II万方数据AbstractTheexpressionstatusandsignificanceofmiR-29aintheperipheralbloodofchroniccoronaryheartdiseasepatientsPostgraduate:XiaoqianHuSupervisor:MingxiZangDepartmentofBiochemistryandMolecularbiologyBasicMedicalSchoolofZhengzhouUniversit

4、yZhengzhou,450001AbstractHeartfailure(HF)isaheartmalfunctionsyndromewhichtriggeredbyvariouskindsofheartdiseases,anditslethalityisseverest.Recently,duetotheaggravationofagingpopulationandtheprevalenceofdiseasessuchashypertension,HFhasalreadybecamethemostimportantheartdiseasewithadramaticallyincreas

5、edmorbidity.ThecommoncausesofHFarecoronaryheartdiseaseandhypertension.InChina,theHFmorbidityrate,causedbycoronaryheartdisease,was36.8%in1980andithasbeenapproached45.6%in2000,suchsituationmakecoronaryheartdiseaseasthemajorcauseofHF.MicroRNAsareabunchofendogenouslyandhighlyconservedsingle-strandedno

6、n-codingsmallRNAswithalengthofapproximate22bp.ThemechanismsofmicroRNAsfunctionaregeneexpressionregulationwhichconductedviatargetingIII万方数据Abstractthe3’-UTRsequenceoftargetgene.Forthepastfewyears,mountingresearchesindicatethatmicroRNAsinvolvesmultiplecardiovasculardiseaseregulationprocesses,anditca

7、nexiststeadilyinthecirculatingblood,therefore,microRNAshasthepotentialstobeadiagnosticbiomarkerofcardiovasculardisease.PPARδ/βisknownasasubtypeofPPARs,recentresearchesindicatethat,PPARδhastheabilitytoregulatemyoc