microrna3383p在结直肠癌中的表达及其临床价值

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1、ExpressionandclinicalvalueofmicroRNA·-338--3pincolorectalcarcinomaName：SuGui．YuanSupervisor：prof．LiGuo·—XinABSTRACTColorectalcarcinoma(CRC)isthethirdmostcommoncancerworldwide谢tllanestimatedonemillionnewcasesandhalfamilliondeathseachyear．Theoverall．．5．．yearsurvivalrateforCRCpatientsislessthan50

2、％inChina．ScreeningforCRCallowsearly．stagediagnosisofthemalignancyandpotentiallyreducesmortalityofthedisease．ThelaterthepatientisdiagnosedasCRC，thepoorertheprognosisis．AlthoughcolonoscopicscreeningforCRCiscurrentlythemostreliablescreeningtool，theinvasivenatureandthecostincurredhavehamperedthewi

3、deapplicationofthisprocedure．Ontheotherhand，thefaecaloccultbloodtest(FOBT)hasthelimitationoflowsensitivityandrequiresmeticulousdietaryrestriction．Thus，thereisapressingneedfornewnon—invasivebiomarkerstoimprovethedetectionofCRC．AlthoughCEAorCA19．9isusefultumormarkerforthedetectionandmonitoringof

4、CRCdevelopment，thesensitivityislessthan50％respectivelyandnon-specific．IfanewtumormarkerhasmoresensitivityandCanbedetectedintheprimarystageofCRC，itispossibletoextrapolatethelikelihoodofamalignantcolorectaltumor．Thus，discoveryofnewtargetmoleculesthatarecriticallyinvolvedinthemajorityofcasesandex

5、pressedspecificallyintumorswillbeessentialtounderstandthemechanismsandimprovetherapeuticinterventionandprognosisofCRC．MicroRNAs(miRNA)representaclassofevolutionarilyconservedsmallABSTRACTnoncodingRNAs(18-25nt)thatcontrolgeneexpressionbytargetingrnRNAandtriggedngeithertranslmionrepressionormRNA

6、degradation，ThemiRNAsplayimportantrolesinthedevelopment，differentiationandfunctionofvariouscelltypes，andinthepathogenesisofvarioushumandiseases，includingcancer．Currently,morettlan800humanmiRNAshavebeenfoundandregisteredinthemiRNAdatabase，miRBase．Strikingly,about30％ofprotein-codinghumantranscri

7、ptsarepredictedtoberegulatedbythesemiRNAs．However，inspiteofthediscovery．ofvariousmiRNAtargetsimplicatedinhumancancer，mostmechanismsbywhichmiRNAsareinvolvedintumorigenesisremaintobeunelucidated．Indeed，theidentificationofcancer-specificmi