17β雌二醇及其代谢产物2甲氧基雌二醇与慢性低氧性肺动脉高压大鼠体内内皮素1及其受体关系的研究

《17β雌二醇及其代谢产物2甲氧基雌二醇与慢性低氧性肺动脉高压大鼠体内内皮素1及其受体关系的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据英文摘要ovariectomy(OVX)group，EHypoxiagroup，FOVX+Hypoxia+E2group，GOVX+Hypoxia+2MEgroup，HOVX+Hypoxiagroup．GroupAandEonlyopentheabdominalcavity，andthensuture．groupB、CandF、Gwereopenedtheabdominalcavity，foundalongtheoviductovarian，ovariesremoved，closetheabdominalcav

2、ity，Usutureoftheskin，thenweregivenE2209／kg／d，2一methoxyestradiol240g／kg／dsubcutaneousinjection．groupDandHwereopenedtheabdominalcavity，foundalongtheoviductovarian，ovariesremoved，closetheabdominalcavity，Usutureoftheskin．theratsofgroupE．E．G．Hwereplacedinthehypoxicch

3、amber，theanaerobictank24hcontinuoussupplyairandnitrogengasatmosphericmixedgas，theoxygenconcentrationofoxygenboxwascontrolledbyoxygenmonitoringcontrolsystem(10+0．5)％，C02concentrationlessthanO．5％；AllTheratswereNormalfeedingoneweek，afterwaitingforwoundhealing，thenp

4、utthemintothesetenvironment．2Long-termchangesinhypoxicpulmonaryhypertensionrats：Meanpulmonaryarterypressure(mPAP)，wasmeasuredbyright-heartcatheterize．tion，rightventricularhypertrophyindex(RVHI)wascalculatedbytheratioofrightventricletotheleftventricleplusseptum，a

5、ndhypoxicpulmonaryvascularremodeling(HPSR)wasobservedbymicroscope．3ThecontentofETAandETBreceptormRNAexpressioninlungtissuewasmeasuredbyRT—PCR．4ThecontentofET．1inthelungtissueproteinlevelswasmeasuredbyradio-immunityassay．Results：1Thegeneralconditionoftherats：Eigh

6、tweekslater，Theratsunderhypoxiaappeardullcolor，Irishdarkred，shortnessofbreathing，eatingless，withThehypoxiatimeprolonged，Theratactivitygraduallydecreased，finallytorest，notdeath．ComparedwithgroupA，groupEandH’Sweightisobviouslylightly，groupF，G’weightislightly，group

7、B，C，D’weightisnotchange．2mPAP，Rightventricularmorphologychangesoflong-termhypoxia万方数据英文摘要pulmonaryhypertensionrats：Bloodvesselwallsinlungsarethickenobviously，smoothmusclecells(SMC)ofpulmonarysmallarteryincreased，themembrane(PAMT)thickeningandluminalnarrowing；and

8、fightventricularhypertrophy，fightventriculehypertrophybecametodevelopsignificantlyaftereightweeksingroupH；thechangeofpulmonaryarterywallstructureingroupFandGwerebetwe