番茄抗病基因ty1的分子标记与其在辅助选择中的应用

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1、河北科技大学硕士学位论文Abstract1’omatoyellowleafcurldise2Lse(TYLCD)isadeVastating击sease，w11ichoccurrediIltomatoproductioniIlrecentyears．砂一Jw2LsmefirstdiscoVeredresistaIlcegenefortomatoyellowleafcurlVims．hlthjs咖dy，resista薹1theterozygous(砂一．，／砂，)，resistaIlthomoZygotes(7弘J／砂-J)a11dsusc印t

2、iblehomoZygotes(巧，-J缈J)areused嬲exper沛entalmat嘶als．TheprimerSarebaSedontllepublishedI强LPmarkerswmchareti曲tlyliIlkedt0砂一，，aIldtherestrictionenzymeall址ysiswasb2Lsed0n廿1ePCR锄pli矗cation疗a邸ents．Tlle咖dygainedTG97CAPSm破eralldMi23CAPSm破er，W}licharetightly1inkedt0resistancegene砂一J．Th

3、etv旧resistancemarkerscandistiIlguishheterozygouSandhomoz)，gous，a11dt11eyareco-dornjnantCAPSmarkerS．(1)TG97CAPSmarker：byPCR锄plificationandenzymesdigested，resiSl：锄thomo巧gouSmat丽aJs印pear398bp，303bpa11d95bpSpecificsegment；resistanthomozygo璐materialsappear303bp锄d95bpspecificsegm

4、ent；Amdsusceptiblehomozygotematerialsappears0111y398bpsegment．PrimersequencesareTG97F(5’一TJ气ATCCGTCGTTACCTCTCCTT-3’)aIldTG97R(5’一CGGATGACTTCAATAGCAATGA一3’)．TherestrictionenzymeuSedintlleeXp丽mentis1的I．(2)Mi23CAPSmarker；byPCR锄plificationand即zymesdigested，resiStanthomo巧gouSmat

5、erialsget402bpand354bpspecificse訇ment；resistaIlthomozygousmaterialsget402bpspeci矗csegment；AndsuSceptiblehomoz)，gotemat商alsget354bpsegment．P曲lersequenCesareMi23F(5’一TGGA从AATGTTGAAr丌CTT1vrG·3’)，Mi23R(5’一GCA1、ACTATATGGCTTGTTTACCC-3’)．T1lerestri嘶onen巧meuSediIlt11eeXperiIIlentis

6、黜a1．Wtllmeexpe血nentalmatI晡alsofdi髋rentsourcesallddi毹rentgeneticbaCkgrouIl也e彘ctiVenessofTG97CAPSm破er趾dMi23CAPSm破erwereproVed．Elecn．0phoresisresultSV耐母thatmeCAPSmarkerST'G97锄dMi23areS诅bil时ande妇陀ctiVeness，aIldTG97CAPSmarkersweremoreclearlyandeaSilyreco嘶zable，Wmchis剐【1idealm冰er

7、sarldcanbeutilizedintllemarker-assistedselection．hltlleF2popllla廿onsoftomat0seedl协g，TG97CAPSm酞erandMi23CAPSm酬kerwe陀uSediIl廿le弱sistedseleCtio玛锄dgetresista呲homoz)rgouSgen呻伊es：15，20，32，33，35，36，41，43，49，58．11le∞白％weregrowniIlthefield锄dselfseed．TheF3liIleso阮IiIledthree900dlille

8、s，w砸chare33·l，35一l，43·1．33-listheulllirnjtedgrowchtype，删劬its，itS伽twei出1609—1809，hi