枝孢菌cladosporium+sp.bio-1的木质纤维素降解特性分析

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1、Abstract3creenlngandgeneticengineeringofmicroorganismtoobtainefficient119nocellulosedegradationstrainsplaysimportantroleinthefieldsofbioenergvandenVlronmemprotection·Bio一1，isolatedfromthesoilofYuelumountain，couldgrowbyuslngalkaliligninasthesolecarbonsource．Combinedwiththeanalysiso

2、fitsculturecharacters,morphologicalcharactersand18srDNAandITSsequences，Bio．1wasidentifiedasamemberofthegenusClndoSpOrium。JhehlghestextracellularandintracellularlaccaseactivitiesofBio．1were241U／Land187U／Lontheeighthday，respectively．When0．5mmoI／Lcopperwasaddedtothecultureonthefourth

3、day，lacccaseactivitiesof3800U／Land5352U／Lwereobtainedontheeighthday，higherthansometypicalfungiusedfor1accaseproduction．Extracellularandintracellularcellulaseactivitiesof51U／mLand2．9U／mLwereobtainedontheeighthday．1wopreliminaryworkforthefurthergeneticengineeringofBio．1werealsocam∞o

4、ut．Mediumswhichcontainedalkalilignin，carboxymethylcellulose-NaandblrcnwoodxYlanassolecarbonsourceswereused，respectivelyandBio．1sporeswere1noculatedwithdifferentconcentrations．AccordingtoBio．1，sgrowthconditions．aslmpleandeffectiVemethodforscreeningthetransformantswhichcoulddegradea

5、lkalilignin，celluloseorxylanrespectivelywasoptimized：theinoculatedsporeconcentratlonoftransformantswaschosenas108perliterandthecolonywhichgrowswellin1essthan60hourswouldbethemutantthathasbetterdegradationabilltv‘hanthewildtype·Thenadual—tagvectorwithenhancedGFP(EGFP)and协秽Dmycf，2Br

6、esistance(细办)wasconstructed，providingapowerfultoolforthescreenlngoftransformantsofBio-1．AfterbeingsequencedandexpressedintheE．coli,therecombinantplasmidwasconfirmedtobeabletoexpresstheEGFPand幼办gene．Thispapercharacteredandanalyzedalignocelluloses—degradingfungus．Thepreliminaryworkf

7、orfurtherdevelopmentandutilizationofthisfunguswasfinished，whichlaidthefoundationforobtainingsuperiorstrainsusedinbioenergyandwastewatertreatmentindUstriesKeyWords：Cladosporium；LignocelluloseConstructionofplamidvectorIIIdegradation；Laccase；Endoglucanase；——．一枝孢菌Cladosporiumsp．Bio．1的

8、木质纤维索降解特性研究目录学位论文原创性声明与论文版权使用说明⋯⋯