烟草青枯病拮抗真菌的筛选、鉴定与活性产物的研究

《烟草青枯病拮抗真菌的筛选、鉴定与活性产物的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、产物的稳定性。发现菌株3F03的活性产物的热、pH和紫外稳定性较好，经蛋白酶K和胰蛋白酶处理抑制率仅分别下降了17．O％、14．5％；菌株7F06的活性产物抗紫外线和蛋白酶的能力较强，酸性环境下抑菌活性仅稍有下降，活性产物经80℃以上高温处理后抑菌能力基本丧失。关键词：烟草青枯病；拮抗真菌；活性产物；鉴定：发酵条件；稳定性ScreeningandIdentificationofAntagonisticFungiagainstRalstoniasolanacearumandResearchontheActivePr

2、oductsAbstractTobaccowiltdiseasecausedbyRalatoniasolanacearumisabacterialdisease，widelyoccurredintropicalandsubtropicalzone．IthashappenedandledtoseveredamageinthesouthoftheYangtzeRiverBasinofChina,suchasSichuan，Guangdone,,Fujian，HunanandGuizhouprovince．Yieldl

3、ossoftobaccocanreachupto80％inmostserioustobaccoregion．Traditionalmethodsofchemicalcontrolhavesevereimpactsonecologicalenvironmentfitswellashighcosts，therefore，rotationandmicrobialcontrolwereprovedtobepromisingmethods．Microbialcontrolhastheadvantageofpollution

4、-freeandlong-term，whichcallfundamentallysolvethedrugresidueinthesoil，drugresistanceofpathogensandotherproblems．Itnotonlybenefitstheenvironments，butalsoturnsecologicalresourcesintovirtuouscircle．InordertoprovidethetheoreticalbasisofbiologicalcontrolofRalatonia

5、solanacearum，thisdissertationfocusedparticularlyonstudiesontheactivestrainscreening,identification,optimizationoffermentationconditions，tobaccoseedlingtestinthepresenceoffermentationbroth，andstabilityofproductsofactivestrains，themainresultsaleasfollow．1．Fifly

6、ty-ninefungusstrainswereisolatedfromsoilsamplesintobaccofieldscollectedfromQianjiangdistrict，Chongqing．PaperdiSkmethodWasappliedtoscreenantagonistfungi，and2stains，named3F03and7F06，behavedstronginhibitionactivities．ThediameterofinhibitionzoneWas10．Ommand9．5mm,

7、usingtheconcentrationof250“gcrudeextractperpiecepaperdiskonplate．Repeatedplateantagonismtestshowedthediameterofinhibitionzonehadnoobviousdifference．2．Accordingtothemorphological，cultural，cellularfattyacidandrDNAITSsequenceanalysis，activestrains3F03and7F06were

8、identifiedasAspergillusfumigatusandNeosartoryaaureola，respectively．3．Thegrowthoftobaccoseedlingstreatedwithfermentationbrothofstrains3F03and7F06wasingoodcondition．Comparedwitllcontrols，th