鸭源抗鹅细小病毒卵黄抗体的制备

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1、AbstractPreparationofDuckImmunogbulinofYolkagainstGooseParvovirusGooseparvovirusInfectionwascausedbyGooseparvovirus，itleadyounggoslingsandmuscovyducklingsundertheagefrom3to25dayoldtodeath．Thediseasehasfastinfected。hilghincidenceandhighfatality,whichinducedacuteorsubacutesepticin

2、fectionsandthecharacterizationofinfectionwasseverediarrhea．GPVisanimportantinfectiousdiseasehazardsingooseindustry．DiseasepreventionandcontrolofgooseparvovirusWasmainlyfocusingonprevention，thespecificantiserumisthetraditionalbiologicalagents，andeggyolkantibody(Immunogbulinofyolk

3、,IgY)wasdevelopedinrecentayearsasanothernewantibodiesincludingchemicallystable，highyield,lowcost,moresuitablefordevelopmentofspecificantibodies．Atpresent，theyolkantibodyWasextractedfromlayinghensandducks，andducksarecloserwithgeeseinaspectsofphylogeny,anatomyandmolecularstructure

4、．Sodevelopingofduckeggyolkagainstgooseparvovirusisimportanttoprovideantibodybiologicalagents．LayingduckswereimmunizedwithGPV98Estrains．Y．olkantibodiesfromduckwereextractedandpurifiedbywaterdilution·sodiumsulfatemethodfromduckyolkafterharvestingduckeggsandseparatingeggyolk．AGPtit

5、er,contentandyieldweredetermined．Preventionandtreatmentweredeterminedamongyounggoslingswithoutmatemalantibodies．Experimentalresultsshowedthatspecificantibodiesofhi【ghlevelsappearquicklyaRerduckswasimmunizedwithlivedGPVfromgoose’Sembryo．TheAGPtiterofthepurifiedyolkantibodiesfromd

6、uckWas1：32，andcontentwas81．6％，andthesumofproteinWas6．01mg／mL．ThefatalityofyounggoslingsWasO％inpreventivetest，andthefatalityofyounggoslingsinjectedantibodiesinpreventivetestdosiswas59％intreatmenttest．Theresultsofsignificancetestshowedthattheantibodiesfromduckyolkhavevalidpreventi

7、veeffectandappropriatetreatmenteffectongoslingsinfectedwithGPV．Keywordsgooseparvovirus；duck；eggyolkantibody；preparation；IgY-111．Candidate：LiuDapengSpecialty：PreventiveVeterinaryMedicineSupervisor：WangJunwei引言1引言1．1鹅细小病毒病研究进展1．1．1鹅细小病毒病概述鹅细小病毒病是由鹅细小病毒(Gooseparvovirus，GPV)引起的急性或亚急

8、性败血性传染病，该病又称Derzsy氏病、鹅细小病毒感染(GooseParvovirusInf