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ID:32237413
大小:2.09 MB
页数:33页
时间:2019-02-02
《大鼠肢体缺血再灌注早期血栓前状态与其干预的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、山西医科大学硕士学位论文大鼠肢体缺血再灌注早期血栓前状态及其干预研究捅要目的观察下肢缺血再灌注早期大鼠血小板膜糖蛋白GPIIb/IIIa和血管内皮细胞血栓调节蛋白(TM)的表达以及经前列地尔(凯时)和肝素干预后的变化,探讨大鼠急性下肢缺血再灌注早期血栓的形成情况,对比前列地尔和肝素对此的干预作用。方法本试验分两部分,第一部分将健康成年雄性sD大鼠随机分为两组:假手术组;下肢缺血再灌注组。夹闭大鼠股动脉并用张力带绑扎下肢,建立急性下肢缺血模型,缺血24,时后恢复血流,3d,时后经心脏取血,用流式细胞技术检测大鼠血小板膜糖蛋自GPIIb/IIIa;取大鼠
2、股动脉,用免疫组化方法定性检测血管内皮TM的表达,并用图像分析系统进行半定量分析。第二部分为药物干预试验,将健康成年雄性SD大鼠随机分为四组:假手术组;下肢缺血再灌注组;下肢缺血再灌注+前列地尔组;下肢缺血再灌注+肝素组。方法同前述,药物组于缺血前经尾静脉给药。结果(1)第一部分:缺血再灌注组血小板膜糖蛋白GPIIb/IIIa表达高于假手术组(P3、P4、f'.■。‘一‘⋯‘。‘●interventionstudyAbstractobjective111eaimofthisstudywastoobservethechangesofplateletmembraneGPIIb/Ⅲaand耶Monvascularendothelialcellinratsafterischemia-reperfusionoflimbandtoobservetheinterventionaleffectsofalprostadilandheparin.Toinvestigatetheformationoft11rombinra5、tsafterischemia-reperfusionoflimbandcontrasttheinterventioneffectofalprostadilwitllthatofheparin.Methodmsstudywasdividedintotwoparts:inthefirstpart,theratsweredividedintotwogroups:shamoperationgroup(Ngroup);thehindlimbischemiareperfusiongroup(IRgroup).Ananimalmodelofacutehindli6、mbischemia-reperfusionWaSdevelopedbyclampingfemoralarteryandbandinghindlimbofrats.ChangesintheexpressionofplateletmembraneGPIIb/IIIaandTMonvaScularendothelialcellinratsafterischemia-reperfusionoflimbwereobserved.Inthesecondpart,theratsweredividedintofourgroups:shamoperationgrou7、p(Ngroup);thehindlimbischemiareperfusiongroup(IRgroup);thellindlimbischemiareperfusionplusheparingroup(mHgroup);andthehindlimbischemiareperfusionplusalprostadilgroup(IRAgroup).Thetreatmentisditto,theadministrationisinjectionthoughcaudalveinbeforeischemia.Result(1)Thefirstpart:T8、heexpressionofGPIIb/ⅢaofplateletmembraneintheIRgroupWa
3、P4、f'.■。‘一‘⋯‘。‘●interventionstudyAbstractobjective111eaimofthisstudywastoobservethechangesofplateletmembraneGPIIb/Ⅲaand耶Monvascularendothelialcellinratsafterischemia-reperfusionoflimbandtoobservetheinterventionaleffectsofalprostadilandheparin.Toinvestigatetheformationoft11rombinra5、tsafterischemia-reperfusionoflimbandcontrasttheinterventioneffectofalprostadilwitllthatofheparin.Methodmsstudywasdividedintotwoparts:inthefirstpart,theratsweredividedintotwogroups:shamoperationgroup(Ngroup);thehindlimbischemiareperfusiongroup(IRgroup).Ananimalmodelofacutehindli6、mbischemia-reperfusionWaSdevelopedbyclampingfemoralarteryandbandinghindlimbofrats.ChangesintheexpressionofplateletmembraneGPIIb/IIIaandTMonvaScularendothelialcellinratsafterischemia-reperfusionoflimbwereobserved.Inthesecondpart,theratsweredividedintofourgroups:shamoperationgrou7、p(Ngroup);thehindlimbischemiareperfusiongroup(IRgroup);thellindlimbischemiareperfusionplusheparingroup(mHgroup);andthehindlimbischemiareperfusionplusalprostadilgroup(IRAgroup).Thetreatmentisditto,theadministrationisinjectionthoughcaudalveinbeforeischemia.Result(1)Thefirstpart:T8、heexpressionofGPIIb/ⅢaofplateletmembraneintheIRgroupWa
4、f'.■。‘一‘⋯‘。‘●interventionstudyAbstractobjective111eaimofthisstudywastoobservethechangesofplateletmembraneGPIIb/Ⅲaand耶Monvascularendothelialcellinratsafterischemia-reperfusionoflimbandtoobservetheinterventionaleffectsofalprostadilandheparin.Toinvestigatetheformationoft11rombinra
5、tsafterischemia-reperfusionoflimbandcontrasttheinterventioneffectofalprostadilwitllthatofheparin.Methodmsstudywasdividedintotwoparts:inthefirstpart,theratsweredividedintotwogroups:shamoperationgroup(Ngroup);thehindlimbischemiareperfusiongroup(IRgroup).Ananimalmodelofacutehindli
6、mbischemia-reperfusionWaSdevelopedbyclampingfemoralarteryandbandinghindlimbofrats.ChangesintheexpressionofplateletmembraneGPIIb/IIIaandTMonvaScularendothelialcellinratsafterischemia-reperfusionoflimbwereobserved.Inthesecondpart,theratsweredividedintofourgroups:shamoperationgrou
7、p(Ngroup);thehindlimbischemiareperfusiongroup(IRgroup);thellindlimbischemiareperfusionplusheparingroup(mHgroup);andthehindlimbischemiareperfusionplusalprostadilgroup(IRAgroup).Thetreatmentisditto,theadministrationisinjectionthoughcaudalveinbeforeischemia.Result(1)Thefirstpart:T
8、heexpressionofGPIIb/ⅢaofplateletmembraneintheIRgroupWa
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