长江上游特有鱼类dna条形码分析

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1、西南大学硕士学位论文intuitiveplotswhen‘comparedwimlinearmodels．Inthepresentstlldy，apartialmitochondrialcytochromeCoXidasesubullitI(COI)genewas锄ployedtobarcodetheendemicfjshes行．omtheUpperYan垂zeRiver．111addition，wetestedmee骶ctiV肌essofCOIgeneforbarcodingofcyprinif．omlfishescombinedourdatawiththedatadownl

2、oaded仃omonlinedatabaserGeI也arlkandBOLD)．Furthermore，SOMmodelwasusedtoValidateitsfeasibilityofpredictingnucleotide—diagnosticsitesinDNAbarcodingresearch。Themainresultsareshownasfollowing：1．Inthepresentstudy，t11reehundredandonelndlVidualsoftheendemicnshesf伯mtheUpperYan昏zeRiVerwerebarcodedusing

3、panialDNAsequenceoftheCOIgene．These301indiVidualsbelongto45species，29genera，8nlrniliesand4orders．Ofwhjch242indiVidualsweresequencedinthepresentstudyandtherestweredownloaded仔omGenBankandBOLD．Theresultsshowedthattheaverage飚mura-s2一par锄eter(K2P)distancewitllinspecies，withingenus，withinf．锄ilyand

4、withinorderwas0．47％，2．60％，16．32％aIld21．76％，respectiVely．Totally，88．89％speciesfomedbarcodinggapbetweenthemaximumintraspeci丘ca11dminimuminterspecificgeneticdistance．√6吼ongthe29genera，speciesin28generaclusteredrespectivelyinmonophyleticcladesontheneighbor-joillingtree，whichconsistedof96。55％ofth

5、etotalcases．HoweVer，speciesinthef01lowingfourgeneraclusterednon—specificmonophyleticclades．ThesespecieSwere(1)&五垃。砍。，谢幽。移∥，sc，)p幻怨p西，&(如Vf讲，S足DZ，DVf，＆g，口矗口朋，Sp，口，2口门ff，SsfnP，堪西andSw口，zgc而f口cJizifingenusSch{zothor(1xjl∞si砼ibotiast译er℃iliarisanaS．}℃eves&e；0)J{扎shni8．abbrIev{口teanaJ．Jf，ze胛s括；(4

6、)尸口，它“cJI￡f^Dg肠n括sfnPnJ如andP口胛抛口胛口，西．Inaddition，．乳凡f6r口，挖口舌口P，zf口f“sand彳c印P行sP，．如6，)w，z托sdidnotfomamonophyleticspecificgroup，respectiVely：Withinthesetaxa，wesuspectedthereasonsmightberapidspeciation，intro孕essiVehybridizationand／orincompletelineagesorting．Tbsumup，theresultsindicatedthatDNAbarc

7、odesbasedonCOIgenewereefrectiVeforrapidandaccurateidentificationofthemajo订tyofmeendemicfishspeciesf酌mtheUpperY妇昏zeRiVerandd啪onstratedmeValidityofSOMmothodforpredictingthenucleotide—diagnosticsitesin加nilyleVelofthese孕oupsoffishes．2．PaItialsequenceso