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ID:32221426
大小:4.80 MB
页数:91页
时间:2019-02-01
《长江上游特有鱼类dna条形码分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、西南大学硕士学位论文intuitiveplotswhen‘comparedwimlinearmodels.Inthepresentstlldy,apartialmitochondrialcytochromeCoXidasesubullitI(COI)genewas锄ployedtobarcodetheendemicfjshes行.omtheUpperYan垂zeRiver.111addition,wetestedmee骶ctiV肌essofCOIgeneforbarcodingofcyprinif.omlfishescombinedourdatawiththedatadownl
2、oaded仃omonlinedatabaserGeI也arlkandBOLD).Furthermore,SOMmodelwasusedtoValidateitsfeasibilityofpredictingnucleotide—diagnosticsitesinDNAbarcodingresearch。Themainresultsareshownasfollowing:1.Inthepresentstudy,t11reehundredandonelndlVidualsoftheendemicnshesf伯mtheUpperYan昏zeRiVerwerebarcodedusing
3、panialDNAsequenceoftheCOIgene.These301indiVidualsbelongto45species,29genera,8nlrniliesand4orders.Ofwhjch242indiVidualsweresequencedinthepresentstudyandtherestweredownloaded仔omGenBankandBOLD.Theresultsshowedthattheaverage飚mura-s2一par锄eter(K2P)distancewitllinspecies,withingenus,withinf.锄ilyand
4、withinorderwas0.47%,2.60%,16.32%aIld21.76%,respectiVely.Totally,88.89%speciesfomedbarcodinggapbetweenthemaximumintraspeci丘ca11dminimuminterspecificgeneticdistance.√6吼ongthe29genera,speciesin28generaclusteredrespectivelyinmonophyleticcladesontheneighbor-joillingtree,whichconsistedof96。55%ofth
5、etotalcases.HoweVer,speciesinthef01lowingfourgeneraclusterednon—specificmonophyleticclades.ThesespecieSwere(1)&五垃。砍。,谢幽。移∥,sc,)p幻怨p西,&(如Vf讲,S足DZ,DVf,&g,口矗口朋,Sp,口,2口门ff,SsfnP,堪西andSw口,zgc而f口cJizifingenusSch{zothor(1xjl∞si砼ibotiast译er℃iliarisanaS.}℃eves&e;0)J{扎shni8.abbrIev{口teanaJ.Jf,ze胛s括;(4
6、)尸口,它“cJI£f^Dg肠n括sfnPnJ如andP口胛抛口胛口,西.Inaddition,.乳凡f6r口,挖口舌口P,zf口f“sand彳c印P行sP,.如6,)w,z托sdidnotfomamonophyleticspecificgroup,respectiVely:Withinthesetaxa,wesuspectedthereasonsmightberapidspeciation,intro孕essiVehybridizationand/orincompletelineagesorting.Tbsumup,theresultsindicatedthatDNAbarc
7、odesbasedonCOIgenewereefrectiVeforrapidandaccurateidentificationofthemajo订tyofmeendemicfishspeciesf酌mtheUpperY妇昏zeRiVerandd啪onstratedmeValidityofSOMmothodforpredictingthenucleotide—diagnosticsitesin加nilyleVelofthese孕oupsoffishes.2.PaItialsequenceso
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