资源描述：
《【5A版】英文-学术报告.ppt》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、CharacteristicsofEscherichiacolibiofilmproduction,genetictyping,drugresistancepatternandgeneexpressionunderaminoglycosidepressureswww.themegallery.comEscherichiacoli大肠杆菌infection感染Aminoglycoside氨基糖苷类antibiotic抗生素Pseudomonas假单胞菌aeruginosa聚合酶链反应技术Crystalvioletstainingmethod结晶紫染色法ph
2、enotype表现型drugresistancepattern耐药模式Antimicrobialagent抗菌药物molecularbiologyreagent分子生物学试剂Brothmicrodilutionmethod肉汤稀释法Susceptibilitytesting药敏试验Genotype基因型Real-timeqRT-PCR实时定量荧光PCRStreptomycin链霉素amikacin丁胺卡那霉素Gentamycin庆大霉素apramycin安普霉素1.Newwords,www.themegallery.comBackgroundSever
3、altypesofinfectioncausedbyEscherichiacoli(e.g.,urethralcatheter,biliarytractprosthesis,biliarytractinfection,lithangiuria,urinarytractinfectionandmanyotherdiseases)areassociatedwithbiofilmformation,whichleadstoaninabilitytoeradicatetheinfectionduetoitsintrinsicnaturetoresisthighl
4、evelsofantibiotics(FontaineandSmith,2006;Melchioretal.,2006)..www.themegallery.comBackgroundInaddition,underextensiveandpersistentpressureofantibiotics,inordertosurvive,theE.coliformsbiofilmtoevadetheimmuneclearanceandresultsinmultipleantibioticsresistance.Hoffmanetal.(2005)found
5、thatsubinhibitorylevelsoftheaminoglycosideantibioticscouldinduceE.coliandPseudomonasaeruginosabiofilmformation.SomeotherstudiesalsorevealedthatbiofilmformationbyE.coliinvitrocorrelatedwiththevirulencefactors(Navesetal.,2008;Rijavecetal.,2008).www.themegallery.comAbstractInthisstud
6、y,qualitative(scanningelectronmicroscope)andsemi-quantitative(modifiedcrystalvioletstainingmethod)methodshadbeenusedtoevaluateEscherichiacolibiofilm-formingability.Brothmicrodilutionmethodandenterobacterialrepetitiveintergenicconsensus-basedPCR(ERIC-PCR)wereperformedtostudyE.colid
7、rugresistancepatternandgenetictyping.Basedontheresultsabove,westudiedthecorrelationbetweenbiofilm-formingabilityphenotype,drugresistancepatternandgenetictypinginE.coli.Real-timeqPCR(qRT-PCR)wasusedtorevealmRNAexpressionlevelofE.colibiofilmrelatedmultipleantibioticsresistancegenes(
8、acrA,agn43,csgA,csgD,ompFandpgaA)underdifferent
