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1、CharacteristicsofEscherichiacolibiofilmproduction,genetictyping,drugresistancepatternandgeneexpressionunderaminoglycosidepressureswww.themegallery.comEscherichiacoli大肠杆菌infection感染Aminoglycoside氨基糖苷类antibiotic抗生素Pseudomonas假单胞菌aeruginosa聚合酶链反应技术Crystalvioletstai
2、ningmethod结晶紫染色法phenotype表现型drugresistancepattern耐药模式Antimicrobialagent抗菌药物molecularbiologyreagent分子生物学试剂Brothmicrodilutionmethod肉汤稀释法Susceptibilitytesting药敏试验Genotype基因型Real-timeqRT-PCR实时定量荧光PCRStreptomycin链霉素amikacin丁胺卡那霉素Gentamycin庆大霉素apramycin安普霉素1.Newwords
3、,www.themegallery.comBackgroundSeveraltypesofinfectioncausedbyEscherichiacoli(e.g.,urethralcatheter,biliarytractprosthesis,biliarytractinfection,lithangiuria,urinarytractinfectionandmanyotherdiseases)areassociatedwithbiofilmformation,whichleadstoaninabilitytoera
4、dicatetheinfectionduetoitsintrinsicnaturetoresisthighlevelsofantibiotics(FontaineandSmith,2006;Melchioretal.,2006)..www.themegallery.comBackgroundInaddition,underextensiveandpersistentpressureofantibiotics,inordertosurvive,theE.coliformsbiofilmtoevadetheimmunecl
5、earanceandresultsinmultipleantibioticsresistance.Hoffmanetal.(2005)foundthatsubinhibitorylevelsoftheaminoglycosideantibioticscouldinduceE.coliandPseudomonasaeruginosabiofilmformation.SomeotherstudiesalsorevealedthatbiofilmformationbyE.coliinvitrocorrelatedwiththe
6、virulencefactors(Navesetal.,2008;Rijavecetal.,2008).www.themegallery.comAbstractInthisstudy,qualitative(scanningelectronmicroscope)andsemi-quantitative(modifiedcrystalvioletstainingmethod)methodshadbeenusedtoevaluateEscherichiacolibiofilm-formingability.Brothmicr
7、odilutionmethodandenterobacterialrepetitiveintergenicconsensus-basedPCR(ERIC-PCR)wereperformedtostudyE.colidrugresistancepatternandgenetictyping.Basedontheresultsabove,westudiedthecorrelationbetweenbiofilm-formingabilityphenotype,drugresistancepatternandgenetict
8、ypinginE.coli.Real-timeqPCR(qRT-PCR)wasusedtorevealmRNAexpressionlevelofE.colibiofilmrelatedmultipleantibioticsresistancegenes(acrA,agn43,csgA,csgD,ompFandpgaA)underdifferent
