黑莓β-葡萄糖苷酶动力学与其花色苷降解机理分析

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1、Blackberrycontainslargeamountofanthocyaninsandhasbeenapotentialsourceofanthocyaninspigment．Consumersarepayingmoreandmoreattentiontoitsanthocyaninsbecauseoftheirpharmacologicalfunctions．However,Anthocyaninsareveryactiveandunstableunderambientcondition，whichleadstothedeg

2、radationofanthocyaninsduetomanyphysicalandchemicalfactors．Forthesereasons，itisnecessarytoexplorethemechanismofanthocyaninsdegradation，whichCalllayfoundationforkeepinganthacyaninsfrombreakingdownandlosingtheirfunction．Thispaperstudiedtheidentificationandcharacterization

3、ofanthocyaninsusingSPEC18columnandI-IPLC—MS／MSmethod．Theenzymolysiscraftoftheblackberrywasalsostudiedbytheresponsesurfacemethodologybasedonthecontentofanthocyanins．TheeffectsofpH，temperature，solublesolidcontent，enzymes，andVitaminConthedegradationofanthocyaninswerestudi

4、ed．Thedegradationmechanismofanthoeyaninsbyfl-glucosidasewasstudied．Theinactivationkineticsoffl-glucosidasebycombinedhighhydrostaticpressureandtemperaturetreatmentsWasalsostudied．1nheresearchresultsasfollows：(1)TheanthocyaninsinblackberrywereisolatedusingHPLCequii；Iment

5、withalichrospherC18columnandaDADdetectorandcharacterizedbyUVspectrometryandelectrosprayionizationtandemmassspectrometry(ESI—MS／MS)AtotalofsevenanthocyaninswerefoundtobeisolatedbytheC18column．Amongthemwerecyanidin(Cy)3一O—glucoside(68．97％)，Cy3-O-arabi-noside(10．44％)，Cy3一

6、O-malonylglucoside(4．50％)，Cy3一O—dioxalylglucoside(12．40％)，Delphinidin(Dp)3一O-arabinoside(2．20％)，Dp3一O-coumaroyld【ucoside(1．18％)andOp3-O—dioxalylglucoside(0．31％)．(2)Asecondorderpolynomialequationfortheenzymolysiscraftoftheblackberrywasestablishedwithresponsesurfacemetho

7、dologybasedonthecontentofanthocyaninsintheblackberryjuice．Thedataindicatedthattheoptimumenzymolysisparameterswere：amountofEnzyme-0．23％：incubationtime-1．49hours；temperature-46．80C．Undertheseconditions，thecontentofanthocyaninsinblackberryjuiceincreasedby32％，whichwasconsi

8、stentwiththemeasuredvalue．Thedegradationprocessofthepurifiedanthocyaninscanbedescribedbythefh-storderkineticmodel，and