白芷的质量控制与体内外代谢分析

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1、inRadixAngelicaeDahuricae．AndtheresultsarehelpfulforUStostartthefurtherstudyofthetransformationpathwaysandexcretionpharmacokineticsofRadixAngelicaeDahuricae．PartOneSimultaneousquantitationofeightcoumarinsRadixAngelicaeDahuricaebymicellarelectrokinetiecapillarychromatographyObjective：Toestablisht

2、hemethodtosimultaneouslydetermineScopoletin，Xanthotoxol，Xanthotoxin，Bergapten，Oxypeucedanin，4英文摘要Imperatorin，CnidilinandIsoimperatorinbymicellarelectrokineticcapillarychromatography．Methods：Separationswerecarriedoutusingfused-silicacapillary(BeckmanTechnologies，USA)of60cmtotallength(50cmeffectiv

3、elength)×75lxmi．d．Therunningbufferwagcomposedof10mmoFLborax，40mmol／Lsodiumdodecylsulfate(SDS)and20％acetonitrile．TheappliedvoltageWaS22kVandthetemperaturewas20℃．田1edetectionwavelengthWag214nm．Results：111e8coumarinswereseparatedsuccessfullywithin20min．Agoodlinearityp≥O．9971)wasfoundintherangeof1．2

4、5-20．04，1．78～28．53，0．44-7．01，0．67-10．74，7．62~121．86，3．94,-63．08，2．89-46．21，3．02,--48．35ng／mLforthe8components，respectively．111erecoverieswerebetween92．7％105．6％，whichweremeettherequirementoftheanalysis．Conclusion：Withthismethod，8coumarinsweresimultaneousdetermined．田1eoperationofthemethodissimple，

5、quick，accurateandCanbeusedasanewmethodforthequalitycontrolofRadixAngelicaeDahuricae．PartTwOThemetabolismofenidilininvitroobjective：Inordertofindthemetabolites。themethodofthemicrobial仃ansformationandtheratintestinalflorawereusedforstudyingthemetabolismofcnidilininvitro．Andthemetaboliteswereobtain

6、edbythepreparative．Methods：1．Themicrobialtransformation：ThemetaboliteswerediscoveredbycomparingtheHPLC-PDAchromatogramsof20fungalstrainssamples谢n1thecorrespondingblanks，andwerepreparedbythefungalwhichcouldtransformthecnidilinthemostcompletely．Themetaboliteswereidentifledby‘H砌andESI．MS，andthechem

7、icalstructuresofthemwereconfirmed。2。砀eratintestinalflora：cnidilinwasanaerobicallyincubatedwithratstoolinvitrotofindthemetabolites．Results：1．朋1emicrobialtransformation：4metaboliteswerefoundby5英文摘要thefungalAS3．970．Twoofthemwer