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ID:31991101
大小:10.04 MB
页数:41页
时间:2019-01-30
《吗替麦考酚酯对stz致糖尿病幼年大鼠肾脏tgf-β1与et-1表达的影响》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、ABSTRACTABSTRACTObjectives:ToresearchtheexpressionsofTransforminggrowthfactor-β1(TGF-β1)andendothelin-1(ET-1)inkidneytissueofJuvenileratsbypreparingtheanimalmodelsofT1DMcausedbystreptozotocin(STZ)andaddingMycophenolatemofetil.Combinedwiththechangeofurinaryprotein,renalfunctionandrenalindex,exploret
2、herelationshipbetweenTGF-β1、ET-1andkidneyinjurycausedbydiabetes.ExploretheprotectiveeffectsofMMFinkidneytissueofdiabeticrats.Method:1.The30maleSDrats,adaptivefeedingoneweek,wererandomlydividedintonormalcontrolgroup(10rats),theexperimentalgroup(20rats).Streptozotocin(STZ),atadoseof65mg/kgbodyweightd
3、issolvedincitratebuffer,wasinjectedintraperitoneallytoinducediabetes.SevendaysafterSTZinjection,bloodglucoselevelwascheckedusingtheglucometer.Theanimalswithabloodglucoselevelofmorethan16.7mmol/Lwereconsidereddiabeticandincludedinthestudy.Theanimalsweresegregatedintothreegroupswithtenanimalseach.The
4、groupsincludedwerenormalcontrolrats(N),STZinduceddiabeticrats(D),diabeticratstreatedwithMMFatadoseof10mg/kg•1daybyfillthestomachfor4weeks(M).TheratsofgroupNandDwerereceivedthesameamountsolventfor4weeks.2.Ratsineachgroupweredealedwithinfourweek.Collectedurinesampleswithmetaboliccagesmethod.Collected
5、bloodandpreparedRenalbiopsy.Bloodglucoselevelwascheckedusingtheglucometer.Serumcreatinine,ureanitrogen,andUrinaryproteinconcentrationweredetectedbyAutomaticbiochemicalanalyzer.QuatilyofkidneyandratweretestedbyElectronicbalance.Renalpathologicalchangeswereobservedunderlightmicroscope(400-fold).TGF-β
6、1,ET-1wasdetectedbyimmunohistochemistry.Result:1.RandombloodglucoseofgroupMandDwereobviouslyincreasedthanIVABSTRACTgroupN(P<0.01),buttherewerenosignificantdifferencebetweengroupMandD(P>0.05).TheKWIofgroupMandDwerehigherthangroupN(P<0.01).Moreover,theKWIofgroupRwerelowerthantheDgroup(P<0.01).2.Scr,B
7、UNand24-hoururineproteinofgroupMandDwereobviouslyincreasedthangroupN(P<0.01),theScr、BUNofgroupMwerelowerthantheDgroup,buttherewerenosignificantdifferencebetweengroupMandD(P>0.05),The24-hoururineproteinofgro
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