怀牛膝连作促进效应与其分子机理.研究

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1、福建农林大学硕士学位论文Positivelyallelopathiceffectandit’SmolecularmechanismofAchyranthesbidenmtaBlume．MediatedbycontinuoumanyyeassoilsABSTRACTcroppingBasedontheinvestigationofapositiveallelopathiceffectofAchyranthesbidentataBlume．inacontinuouscroppingsysteminHenanprovince，anexper

2、imentWaSconductedtostudytheeffectandinfluenceofcontinuouscroppingsoilsofAchyranthesbidentataBlume．．First，theallelochemicalsintherhizosphericsoilsampledfromthefieldofcontinuouslycroppedA．bidentataBlume．plantswereextractedbywaterandorganicsolvent．Theresultsfromthebioassay

3、inPetridishesshowedthatthepromotioneffectoftheextractsonthegrowthofLactucasativaWassignificant，theeffectrates(ERs)ofthetreatmentsweremorethan25％．Soilsofdifferentcontinuouscroppingyears，especiallythetreatmentsofwaterextractionweremorethan100％．Thisresultimplyingthattheall

4、elochemicalsespeciallyfromthewaterextractsmightcontainplantactivatorstopromotethegrowthofL．sativa．Tofurtherunderstandthemechanismofthegrowthpromotionbytheextracts，subtractivehybridizationsuppression(SSH)WasusedtoinvestigategeneexpressionprofilesofA．bidentataBlumemtreate

5、dwiththeextracts．TenupregulatedgenesfromSSH—eDNAlibraryweresequencedandassigned．Accordingtothefunctioncategory，theidentifiedgeneswererelatedtotheelementarymetabolism，flavonoidsandphytosterolsynthesis．Proteomicswereemployedtoconfirmthemolecularmechanismofgrowthpromotionb

6、ytheextracts．Theresultshowedthat25proteinschangedintheexpressionabundancesinthetreatedplantscomparedwithcontr01．ofwhich15wereup．regulatedand10down—regulated．Basedonthefunctionsoftheidentifiedproteins，theseprotainsweremainlyrelatedtonutritionabsorptionandmetabolism，Secon

7、darymetabolismandresistance，signaltransduction，aswellasnucleotidesandproteinsynthesis．Basedonanalysisofdifferentialgeneexpressiononandproteinsynthesis，bySSHandproteomics，ThefindingssuggestedthattheformercropA．bidentataBlume．formedallelochemicals，thisallelochemicalsmaybe

8、terpenesorflavonoids，andliberatethoseallelochemicalstoitsgrowthenvironment(bythansformationandamplicationeffec