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《甘蔗roc16的转基因研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、甘蔗ROC16的转基因研究GenetransfertosugarcaneROC16摘要甘蔗(SaccharumofficenarumL.)是我国最主要的糖类经济作物，其生产率的大小将直接影响到蔗农的收入和国民经济的稳定发展。但是在实际的甘蔗生产过程中，各种病虫害的侵害将大大降低甘蔗的产量和品质。在众多的病虫害中，常见的甘蔗害虫是甘蔗螟虫（PiatraeaSaacharalisF.）也是最主要的虫害。本研究在于将修饰的豇豆胰蛋白酶抑制剂基因（sck）及苏云金杆菌毒蛋白基因（Bt）导入甘蔗ROC16种
2、质中。探索获得了农杆菌介导ROC16转基因研究的相关科学数据：诱导甘蔗ROC16愈伤组织的最佳2，4-D浓度为2.5mg/L（诱导率为91.7%）介于ROC16愈伤组织会因2，4-D浓度过高而不易诱导芽分化，在实际的愈伤诱导中，采用了1mg/L（诱导率为72.9%）。采用2mg/L6-BA+1mg/LKT+200mg/Lcar进行芽的高浓度培养基中诱导刺激分化15天，再转入0.5mg/L6-BA+0.25mg/LKT+200mg/Lcar的低浓度培养基中15天，最后转入1mg/L6-BA+0.5m
3、g/LKT+200mg/Lcar培养基中持续分化，可解决ROC16在常规浓度下（1mg/L6-BA+0.5mg/LKT+200mg/Lcar）难以诱导芽分化问题。关键词：甘蔗；ROC16；农杆菌；转基因；sck基因；Bt基因ABSTRACTThesugarcaneisthemostmaincarbohydrateindustrialcropinmycountry,Itsproductivitywilldirectlyaffectthesizeofnationaleconomicincome/sta
4、bledevelopment,Butinactualsugarcaneproductionprocess,variousinsectviolationswillgreatlyreducethesugarcaneyieldandquality,Inmanyoftheinsectpest,commoncanecaneisstemborerandoneofthemostimportantpests.ThisresearchistomodifycowpeatrypsininhibitorSCKgenean
5、dgeneROC16sugarcaneBtgermplasm.TheexplorationobtainedtheagriculturalbacillustoliebetweenleadstheROC16transgeneresearchtherelatedscientificdata:InductionsugarcaneROC16injuriestheorganizationthebest2,4-Ddensityis2.5mg/L(inductivityis91.7%),Roc16betweenc
6、allusbecause2,4-dhighconcentrationandeasilyinducedbudpointsactualinjuriesintheinduction,hasused1mg/L(inductivityis72.9%).Using2mg/L6-BA+1mg/LKT/caron200mg+highconcentrationsofbuddifferentiationmediuminducedstimulusfor15days,Againinto0.5mg/L6-BA+0.25mg
7、/LKT200mg+/caroflowconcentrationmediumfor15days,Finally1mg/Lto6-BA+0.5mg/LKT200mg+/carmediumcontinuousdifferentiation.ROC16cansolveinconventionalconcentration(1mg/L6-BA+0.5mg/LKT/car)200mg+toinducebuddifferentiationquestion.ButafterROC16genetictransfo
8、rmationtoprovidethescientificbasis.Keywords:Sugarcane,ROC16,Agrobacterium-mediated,transgenic,sckgene,Btgene目录1前言11.1甘蔗概况11.2甘蔗遗传转化国内外研究进展12材料与方法32.1材料32.1.1植物材料32.1.2菌种和质粒32.1.3试剂32.1.4主要仪器设备32.2方法32.2.1技术路线32.2.2cpti基因的修饰42.2.3植物表达载体的构建42.2.
