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1、5种石斛对四氯化碳致小鼠急性肝损伤的保护作用［摘要］❷文旨在研究比较霍山石斛、铁皮石斛、铁皮石斛、铜皮石斛和河南石斛等5种石斛对四氯化碳急性肝损伤的保护作用。实验将C57BL/6小鼠随机分为空白对照组、模型对照组、5种石斛高和低2个剂量组。各组连续给药2周，末次给药2h后，除对照组腹腔注射橄榄油外，其余各组腹腔注射0.5%CC14橄榄油溶液。16h后，采集小鼠血清及肝组织，测定小鼠血清中ALT,AST的活性；并检测肝组织中SOD和MDA的水平；计算肝脏指数并观察肝组织病理学变化，荧光定量PCR技术检测肝组织中IL-ip,IL-6和TNF-a的mRNA的表达水平
2、。结果显示，与模型组相比，各石斛组均能降低小鼠血清指标ALT和AST水平，使肝组织中MDA含量下降，SOD活性升高，减轻CC14对肝脏的病理损伤。此外，霍山石斛肝组织中IL-ip,IL-6和TNF-a的mRNA表达水平显著下调。结果表明，5种石斛对四氯化碳急性肝损伤具有保护作用。但不同产地石斛和不同种石斛的保肝效果有所不同，其中霍山石斛效果最为显著，其他几种石斛保肝效果依次为铁皮石斛、铁皮石斛、河南石斛和铜皮石斛，这可能与不同基源品种石斛化学成分不同有关。[关键词]石斛；保肝；四氯化碳；急性肝损伤[Abstract]Thisstudyaimstoinvesti
3、gatetheprotectiveeffectofDendrobiumhuoshanense,D.officinale,D.officinale,D.monilifbrmeandD.henanenseonCC14-inducedhepaticdamageinmice.C57BL/6micewererandomlydividedintocontrolgroup,modelgroup,high-doseandlow-dosegroupsofthefiveDendrobium.Eachgroupwasintragastricallyadministeredwithd
4、rugsfor2weeks・ThecontrolgroupwasintraperitoneallyinjectedwithOliveoilsolution,whiletheothergroupswereintraperitoneallygiven0.5%CC14combinedwithOliveoilsolution2hlaterafterthelastadministration.Subsequently,ALTandASTactivitiesinserum,SODactivitiesandMDAcontentsinlivertissuesweredeter
5、minedinallgroups16hlaterafteradministration・Theliverindexwascalculated,andhepatichistopathologicalexaminationwasperformed・ThemRNAexpressionsofIL-1卩,IL-6andTNF-awereanalyzedbyReal-timePCR・ComparedwiththeCC14modelgroup,theactivitiesofALTandASTinserumdecreasedsignificantlyinthefivediff
6、erentDendrobiumgroups.Meanwhile,inlivertissues,thelevelsofMDAreducedobviously,whiletheSODactivitiesmarkedlyincreased.Furthermore,livertissuedamageinducedbyCC14wasamelioratedaccordingtothehistopathologicalexamination.IL-lp,IL-6andTNF-amRNAexpressionsinD.huoshanense-treatedlivertissue
7、sweresignificantlydecreased.Inconclusion,thefivedifferentDendrobiumgroupsshowedhepatoprotectiveeffectsonCC14-inducedacuteliverinjuryinmice・However,thereweredifferencesamongDendrobiumofdifferenttypesandorigins.TheprotecteffectofD.huoshanenseisthemostobvious,andtheorderoftheprotective
8、effectoftheotherDen
