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1、液相色谱分析实验LiquidchromatographyexperimentI.e.Understandandunderstandtheprocessofhigh-performanceliquidchromatograph・Graspthequalitativeandexternalquantitativemethodofliquidchromatography.Understandtheclassificationofliquidchromatographyandchromatographiccolumn.Two,[experimenta
2、lprinciple.Thetheoryofseparationofliquidchromatography:WhereKisthedistributioncoefficient,Kprimeisthecapacityfactor,Vm.Visthevolumeofthemovingphaseandthefixedphaserespectively,andthepandqaretheweightsinthestationaryphaseandthemovingphaserespectively.Benzene,naphthaleneandbi
3、phenyl,Philippinesrespectivelybelongtothering,polycyclicaromatichydrocarbonsandpolycyclic,andtheintensityofpolarityandmolecularweighthasthedifferenee,therefore,theirflowfaciesinstronglypolarnon-polarstationaryphasedistributioncoefficientKisdifferentbetweentheretentiontimeis
4、notthesame,mixedsamplesandcanbeseparatedinthechromatographicsystem・Becauseofbenzene,naphthslene,andbenzobenzene,itisabsorbedbyultravioletwavelengths,sotheuvdetectoristested.Inthednti-phasechromatographicsystem,thestationaryphaseisnonpolarandtheflowispolar.Inchemicalbondsand
5、chromatography,theligandofafixedphaseisoftenthealkylof2-18carbonatoms・Theflowphaseisusuallyamixtureofpolarorganicsolventsandwater・Intheorderofdissolutepolaritytheorderiswashedaway.Asthemovementofthepolarityincreases・Theretentionvalueincreasesaswel1.Reversed-phasechromatogra
6、phicseparationishydrophobicsolutestructuredifferences,onthebasisofthepolarityofthesolute,thegreatertheretentionvalueissmaller,thehomologue,itsretentionvalueincreaseswiththeincreaseofcarbonnumber・Solventcompositionofthesampleretentionhasaveryprofoundeffect,thesizeofthesolute
7、retentionisdecreaseswiththedecreasingofthepolarityoftheeluent,whenthemobi1ephasecompositionofacertainsampleoveralongeralkylligandlargeretainedonthestationaryphase・Inhighperformanceliquidchromatography(HPLC),thequalitativemethodwithknownstandards,retainvalueexperiencequalita
8、tiveandqualitative,withdifferentchromatographicsystemwithothermethodssuchasqualita
