资源描述:
《急性脊髓损伤后脊髓组织内皮素-1mrna表达的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、急性脊髓损伤后脊髓组织内皮素-ImRNA表达的实验研究Anexperimentalstudyoftheexpressionofendothelin-lnrrnainthespinalcordafteracutespinalinjuryUpdatedate:2011-05-11lukwuqiangjiangyingMingAbstract:objectivetoexploretheexpressionandmechanismofendothelial-1(et~l)inacutespinalcordinjury.Methods35gloadwasapplied
2、tothespinalcord(T8、9)5minofratthorax,causingacuteandmoderateinjurytothespinalcordofrats・ThetimeandspatialdistributioncharacteristicsoftheearlytTmRNAexpressionwerestudiedbymeansofthechemicalmethodofin-situhybridizationandthequantitativestudyofthetimeandspatialdistributionoftheearlyt
3、~lmRNAinratswithacutespinalcordinjury.Results30minET-1mRNAwassignificantlyincreasedafterthespinalcompressioninjury,andthepeakof4hreachedthehighestpeak,whichlastedto72handreturnedtonormaLET1mRNAexpressionpositivecellsmostlyneurons,highexpressioninthespinalcordanteriorhorn(IXarea)・Co
4、nclusionthecontentofET-1inthespinalcordtissueafterinjuryisoneofthemainreasonsbecauseofitsabnormalexpressionoftranscription,ET-1isanimportantdamageofspinalcordsecondaryinjuryfactors・Keywords:spinalcordinjury;Endothelial一1;RNA,messenger;InsituhybridizationTheExperimentalstudyonexpres
5、sionofendothelin一1mRNAinspinalcordinjuryLUKaiwu,HOUTiesheng,FUQiang,etal.(Dept.OfOrthopedics,ChanghaiHospital,TheSecondMilitaryMedicalUniversity,Shanghai200433,China)Abstract:ObjectiveToexploremRNAexpressionandactionmechanismofendothelin-1(ET-1)inspinalcordinjury(SCI)・MethodsThetho
6、racicspinalcord(T8-9)ofratswascompressedbyaloadof35gfor5minutes,whichcausedanacuteandmoderateinjury.Bymeansofinsituhybridizationhistochemistry(ISHH),thedistributiveparticularitiesintimeandlocationoftheearlyexpressionofET-1messengerribonucleicacid(ET-1mRNA)ininjuredspinalcordtissues
7、fromratswerequalitativelyandquantitativelymeasured.ResultsTheexpressionofETTmRNAincreased30minutesafterspinalcordcompressionandreacheditsclimaxat4hours・Then,itdecreasedslowlytonormallevelat72hours.ThepositivecellsoftheET-1mRNAexpressionwerelocalizedtoneuronalcellbodiesinspinalcord・
8、Thehighlyexpressiveregions