原发性iga肾病中微小rna的差异表达研究

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1、原发性IgA肾病中微小RNA的差异表达研究眭维国，蓝慧娟，晏强，邹贵勉，李飞，郑艺花，黄河，陈洁晶，戴勇（中国人民解放军第181医院肾脏科，广西桂林541002）摘要：目的借助微小RNA（microRNA，miRNA）芯片研究IgA肾病（IgAnephropathy，IgAN）中miRNA差异表达。方法收集11例IgAN患者肾穿标本作为IgAN组，3例镜下病理检查正常的肾皮质作为对照组。使用Trizol提取每组总RNA,分离miRNA，采用miRNA芯片筛选出差异表达显著的miRNA，实时定量PCR技术验证芯片结果可靠性。结果IgAN中miRNA出现显著差异表达65个，其中

2、显著表达上调34个，显著表达下调31个。挑选hsa-miR-637、hsa-miR-492进行实时定量PCR相对定量检测，结果显示2种方法检测的miRNA表达量和表达趋势相符，芯片结果具有可靠性。结论IgAN是一种经典的多因素、多基因病，目前其机制尚未明确。本实验表明miRNA在IgAN中出现显著差异表达，深入研究将有助于IgAN机理研究及其诊断、治疗和预防。关键词：微小RNA表达；微小RNA芯片；IgA肾病中图法分类号：文献标识码：ThestudiesofMicroRNAsdifferentialexpressioninIgAnephropathyWeiguoSui,Hu

3、ijuanLan,QiangYan,GuimianZou,FeiLi,YihuaZheng,HeHuang,JiejingChen,YongDai（KidneyTransplantationandHemoPurificationCenter,181HospitalofGuilin,GuangxiProvince,541000,China）Abstract：ObjectiveStudiedmicroRNAsdifferentialexpressioninIgAnephropathyusingmicroRNAsarray．MethodsCollectedbiopsiesof11

4、patientswithIgAnephropathyasIgAnephropathygroupand3renalcortexesoftissueeumorphismpatientsasnormalcontrolgroup.TotalRNAofeverygroupwasextractedusingTrizolaccordingtothemanufacturer’sinstruction.MicroRNAswereisolatedfromtotalRNAandsignificantdifferentialexpressionmicroRNAswerescreenedbymicr

5、oRNAsarray.ThereliabilityoftheresultsofmicroRNAsarraywasvalidatedbyRealtimePCR.ResultsInIgAnephropathy,therewere65microRNAssignificantdifferentialexpressionofwhich34up-regulatedand31down-regulated.Hsa-miR-637，hsa-miR-492wereselectedforRelativequantificationbyRealtimePCR.Itshowedthattheresu

6、ltsofbothmethodswerenotsignificantdeviationandtheresultsofmicroRNAsarraywasreliable.ConclusionIgAnephropathywasaclassicmultiplefactors,polygenicdiseaseanduntilnowtherewasnomechanismwhichcanidentifyitperfectly.OurdataindicatedthatmicroRNAsweresignificantdifferentialexpressioninIgAnephropath

7、y.ThelucubratedofmicroRNAsinIgAnephropathymayhelptodiagnosis,treatandpreventIgAnephropathy.Keywords:microRNAsexpression;microRNAsarray;IgAnephropathy原发性IgA肾病（IgAnephropathy，IgAN）是世界上尤其是亚洲地区最常见的原发性肾小球疾病，是一种IgA沉淀于肾小球系膜区而造成的肾小球疾病。原发性IgAN具有种族地域差异且临床表现多样，预后相差悬殊，在确诊