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1、重庆地区羊传染性脓疱与病原菌混合感染的检测与分析许国洋1，付利芝1，徐登峰1，龙小飞2，陈朝洪2，李龙2，张素辉1*（1.重庆市畜牧科学院，重庆荣昌402460，2.重庆市武隆区畜牧兽医局，重庆武隆408500）摘要：采集山羊口唇部痂皮，通过PCR检测、MDBK细胞培养、电镜观察、间接免疫荧光（IFA）检测对羊传染性脓疱病毒进行系统鉴定，同时，分离培养病灶部位主要病原菌，进行分子鉴定、致病性和药敏试验检测，初步分析病原特性。结果表明，共分离到1株羊传染性脓疱病毒，3种病原菌，通过对病原菌16Sr
2、DNA基因序列比对分析，发现其分别与多动物链球菌、金黄色葡萄球菌和溶血性曼氏杆菌同源性较高，在系统进化发育树中分别与其聚为一簇。致病性分析，发现多动物链球菌和溶血性曼氏杆菌对小鼠的致死率高达100%。3种病原菌主要侵染部位为小鼠心脏、肝脏和肺脏，能够引起肺脏和肝脏明显病变。药敏试验表明，3种菌均对舒巴坦、阿米卡星和培氟沙星等6种药物高度敏感。本研究首次从羊传染性脓疱病变部位分离到多动物链球菌、金黄色葡萄球菌和溶血性曼氏杆菌，为该病在重庆地区的流行特点和防治技术研究提供了理论依据。关键词：羊传染性
3、脓疱；病原菌；混合感染；多动物链球菌；金黄色葡萄球菌；溶血性曼氏杆菌中图分类号：S827；S855文献标识码:ADetection and analysisofmixedinfectionofcontagiousecthymaandpathogenicbacteriaingoatsofChongqingXUGuo-yang1,FULi-zhi1,XUDeng-feng1,LONGXiao-fei2,CHENChao-hong2,LILong2,ZHANGSu-hui1*（1.Chongqinga
4、cademicofscienceandtechenology,Chongqing402460,China;2.WulongDistrictBureauofAnimalHusbandry,Chongqing408500,China）Abstract:PCRdetection,MDBKcellculture,electronmicroscopyandindirect immunofluorescence(IFA)werecarriedoutfor the systematic identificat
5、ion ofcontagiousecthymaingoats.Thepathogenswereisolatedandcultured,identifiedbymolecular identification,pathogenicanalysisanddrugsensitivitytestatthesametime.Theresultshowedthatone virus strainandthreekindsofpathogenicbacteriaweresuccessfullyisolated
6、.Thepathogenic bacteriarespectively wereStreptococcuspluranimalium,Staphylococcusaureus,Mannheimiahaemolyticaaccordingtothe16SrDNAidentification.Andeachpathogenicbacteriaformedaseparatewith thecorrespondingstrainsaccordingtothesystemdevelopmentofthee
7、volutionarytree,respectively.TheresultofpathogenicanalysisshowedthatthefatalityrateinmiceofStreptococcuspluranimaliumandMannheimiahaemolyticawereashighas100%.Themaininfectionsitesofthethreepathogensweremouseheart,liverandlung,whichcouldcauseobviousle
8、sionsinthelungsandliver.Thedrugsensitivitytestshowedthatallthreestrainswerehighlysensitivetosixdrugs,suchassulbactam,amikacinandpefloxacin,etal.Streptococcuspluranimalium,StaphylococcusaureusandMannheimiahaemolyticaweresuccessfullyisolatedforthefirst
