各种浓度各种ph的pbs、tris-hcl缓冲液配制

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1、一、PBS缓冲液1.1母液的配制：0.2MNa2HPO4：称取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：称取31.2gNaH2PO4-2H2O，溶于1000ml水1.2不同PH值PBS配制各种PH值的0.2MPBS（100ml)配方：pH    0.2MNaH2PO4（ml）      0.2MNa2HPO4（ml）5.7        93.5                  6.55.8        92                    8        5.9        90          

2、          106.0        87.7                  12.36.1        85                    156.2        81.5                  18.56.3        77.5                  22.56.4        73.5                  26.56.5        68.5                  31.56.6        62.5                  37.56.7       

3、 56.5                  43.56.8        51                  496.9          45                  557.0          38                  627.1          33                  677.2        28                  727.3          23                  777.4          19ml                81ml7.5      

4、    16                  847.6          13                  877.7        10.5                  0.57.8          8.5                  91.57.9        7                    938.0        5.3                  94.7以配制100ml0.2MPBS为例，先配制母液，按照配方表分别取19ml0.2mol/L的NaH2PO4和81ml0.2mol/L的Na2HPO4,

5、混合即可。1.3不同浓度PBS的配制只需将0.2MPBS按相应比例适当稀释即可，如：0.1MPBS（PH=7.4）：取500ml0.2MPBS，加水稀释至1000ml即可。0.01MPBS（PH=7.4）：取50ml0.2MPBS，加水稀释至1000ml即可。0.02MPBS（PH=7.4）：取100ml0.2MPBS，加水稀释至1000ml即可。若需要NaCl的话，加入NaCl至0.9%（g/100ml）即可。 二、Tris-HCl缓冲液  某一特定pH值的0.05mol/LTris缓冲液的配制：将50ml0.1mol/LTris碱溶液与配方表中所示

6、相应体积（单位：ml）的0.1mol/LHCl混合，加水将体积调至100ml即可。(至于配制0.1MHCl就是8.58ml浓盐酸用蒸馏水定容到1000ml啦)各种ph值的tris缓冲液的配制所需ph值（25℃）0.1mol/lHCl的体积/ml7．145．77．244．77．343．47．442．07．540．37．638．57．736．67．834．57．932．08．029．28.126.28.222.98.319.98.417.28.514.78.612.48.710.38.88.58.97.0