严格厌氧发酵体系生产丁醇及在线尾气监测系统的设计-生物学专业毕业论文

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1、严格厌氧发酵体系生产丁醇及在线尾气监测系统的设计高19．O％，并且丁醇丙酮比相对于对照组的2．45提高了0．36。游离发酵的发酵周期为44h，固定化发酵周期明显缩短了，丁醇的生产强度显著提高。固定化发酵周期为32h，丁醇最高产量为15．4g／L，生产强度0．64g／(L·ho)，分别比对照组提高了12．4％和106％，C02和H2最高产气速率分别提高了60％和9％，产气量分别提高了20．7％和41．3％。加入中性红的固定化发酵，丁醇和ABE的最高产量为16．8g／L和26．8g／L，丁醇产量和ABE产量分别

2、提高了22．6％和25．8％。用酸水解和酶水解的方法水解海带，我们可以建立海带生产甘露醇和还原糖的最佳条件。最佳的酸解条件为底物浓度为8％、酸解时间为90min、酸解温度为120℃、酸浓度为0．5M。对酸解后的水解液进行酶解，甘露醇和还原糖的含量都有所提高。以海带水解液为底物发酵产丁醇，总糖23g／L，丁醇产量为1．2g／L，得率为0．05g／g关键词：丁醇；厌氧发酵；尾气在线监测；海带万方数据大连理工大学硕士学位论文ButanolProductioninAnaerobicFermentationSyste

3、mandTheDesignofOnlineOff-gasAnalysisforAnaerobicFermentationAbstractButanol，isnotonlyanimportantorganicchemicalrawmaterials，butalsoanewbiofuelwithgreatpotential．However，usingtheconventionalacetone-butanol-ethanol(ABE)fermentationtoproducebio-butanolhassome

4、questions．Duetothepresenceoftoxicofbutanolinhibition，itwillresultinfinallowproductconcentrationandlowproductivity．Inaddition，thehighcostofrawmaterialsisanotherfactorinthedevelopmentofbio-butan01．Thenanonlineoff-gasanalysisforanaerobicfermentationcanbedesin

5、ged．Usingtheonlineoff-gasanalysis，thefermentationperformanceofCacetobutylicumwereinvestigated．Theonlineoff-gasanalysissystemcanprovidedetailedgasdatastoanalysisanaerobicmetabolicprocess。Addingneutralredandusingimmobilizationtechnologycanproduceahighconcent

6、rationandhighproductivitybutan01．Seaweed，isarawmaterials。Amethodofpretreatmentandenzymaticsaccharificationtechnologyhasbeenestablished．Thenwecanexplorethecontentofreducingsugarsandmannitolinseaweed，toconductafeasiblestudyofitsenergy．Thedesignofanaerobicfer

7、mentationoff-gasonlinesystemisfeasible．Thevolumeofoxygeninthissystemisonly5．9mL，thevolumeofoxygeninaerobicfermentationoff-gasonlinesystemis123．7mL．Theaerobicfermentationoff-gasonlinesystemmonitoringtotalgasvolumewas21．0L，H2was7．4L，C02was12．5L．Theanaerobicf

8、ermentationoff-gasonlinesystemmonitoringtotalgasvolumewas34．9L，H2was13．9L，C02was19．3L．Thetotalgasvolume，C02volumeandH2volumeincreasedby66．2％，87．8％and54．4％inanaerobicfermentationoff-gasonlinesystemrespectively