欢迎来到天天文库
浏览记录
ID:28844514
大小:10.93 MB
页数:67页
时间:2018-12-14
《基于细胞芯片平台的量子点细胞毒性分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、摘要AbstraetQuantumdots(QDs)areregardedasPromisingfluorescentProbesforbiologicalaPPlieationsduetouniquesize一dePendentoPticalandelectronieProPerties.ComParedwithtraditionalorganiefluoroPhores,QDshavealotofadvantagesineludinghighfluoreseeneeintensity,broadabsor
2、Ptioneoeffieients,narrowemissionsPectra,sizedePendingemissionandhighPhot0Stability,WiththeaPPlicationandinvestigationofthem,theresearehoftheircytotoxieitybeeomesmoreandmoreimPortant.Mierofluidics15agrowingtechnologywidelyusedinbiologyandehemistryareas.Amier
3、ofluidieehiPeaneombinelotsofstePsontoasingledeviee.Forlifeseieneestudy,mierofluidiceellehiPssuPPlyabetterwayforeell一basedresearehorassay.WithmierofluidicchiPs,alotofdetaileellanalysishavebeenrealizedeasily.Inthisstudy,wedemonstratedmierofluidiechiPasaPowerf
4、ultooltoevaluatethecytotoxicityofQDs,sPeeificallythewater一disPersedCdTe,CdTe/CdSandCdTe/CdS/ZnSQDsinHEK293cells.1,ThemasterofmierofluidicchiP15fabricatedbySU一8一075PhotoresistonsilieonwaferandtheehiP15madeofPDMS.MierofluidicehiPssuPPliedexeellentobservation,
5、fluoreseeneedeteetioneonditionsandhighthroughPutanalysis.2.ThemicrofluidieehiP15linkedtoaeellcultureequiPments(airandheatsuPPort)andsuPPlieseontinuousreal一timeobservationwithmieroseoPeforalongtime.3.Weanalyzedtheeellularmorphologyandcellsurvivalratewith3kin
6、dsofQDsinconcentrationgradientanddifferentaPPlicationtime.CdTeQDseausedcelldamagewiththeeoneentrationover0.5群Min24handtheeytotoxicityofCdTeinereasedwithconeentrationandeffeettime.CdTe/CdSshowhigheytotoxicityafter72heffeettime,whileCdTe/CdS/ZnSshowlittlecyto
7、toxieitywithhigheoneentration(1召M)andlongeffecttime(72h).4.TheresultsofeelldivisionratewithQDsareobtainedbystainingthecellswithfluoreseentdye.WefoundthateomParedtotheothertwokindsofQDs,CdTeinhibitedcelldivision.5.WithCaleeinAMandHoehest33342doublestain主ng,t
8、heinformationabout摘要QDsaeeumulationanddistributionineells15obtained.TheinformationofeellnueleimorPhology,eellaPoPtosisandQDsdistributionineellseanbecolleetedalltogetheratthesametimebythismethod.Inourst
此文档下载收益归作者所有