基于细胞芯片平台的量子点细胞毒性分析

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1、摘要AbstraetQuantumdots(QDs)areregardedasPromisingfluorescentProbesforbiologicalaPPlieationsduetouniquesize一dePendentoPticalandelectronieProPerties.ComParedwithtraditionalorganiefluoroPhores，QDshavealotofadvantagesineludinghighfluoreseeneeintensity，broadabsor

2、Ptioneoeffieients，narrowemissionsPectra，sizedePendingemissionandhighPhot0Stability，WiththeaPPlicationandinvestigationofthem，theresearehoftheircytotoxieitybeeomesmoreandmoreimPortant.Mierofluidics15agrowingtechnologywidelyusedinbiologyandehemistryareas.Amier

3、ofluidieehiPeaneombinelotsofstePsontoasingledeviee.Forlifeseieneestudy，mierofluidiceellehiPssuPPlyabetterwayforeell一basedresearehorassay.WithmierofluidicchiPs，alotofdetaileellanalysishavebeenrealizedeasily.Inthisstudy，wedemonstratedmierofluidiechiPasaPowerf

4、ultooltoevaluatethecytotoxicityofQDs，sPeeificallythewater一disPersedCdTe，CdTe/CdSandCdTe/CdS/ZnSQDsinHEK293cells.1，ThemasterofmierofluidicchiP15fabricatedbySU一8一075PhotoresistonsilieonwaferandtheehiP15madeofPDMS.MierofluidicehiPssuPPliedexeellentobservation，

5、fluoreseeneedeteetioneonditionsandhighthroughPutanalysis.2.ThemicrofluidieehiP15linkedtoaeellcultureequiPments(airandheatsuPPort)andsuPPlieseontinuousreal一timeobservationwithmieroseoPeforalongtime.3.Weanalyzedtheeellularmorphologyandcellsurvivalratewith3kin

6、dsofQDsinconcentrationgradientanddifferentaPPlicationtime.CdTeQDseausedcelldamagewiththeeoneentrationover0.5群Min24handtheeytotoxicityofCdTeinereasedwithconeentrationandeffeettime.CdTe/CdSshowhigheytotoxicityafter72heffeettime，whileCdTe/CdS/ZnSshowlittlecyto

7、toxieitywithhigheoneentration(1召M)andlongeffecttime(72h).4.TheresultsofeelldivisionratewithQDsareobtainedbystainingthecellswithfluoreseentdye.WefoundthateomParedtotheothertwokindsofQDs，CdTeinhibitedcelldivision.5.WithCaleeinAMandHoehest33342doublestain主ng，t

8、heinformationabout摘要QDsaeeumulationanddistributionineells15obtained.TheinformationofeellnueleimorPhology，eellaPoPtosisandQDsdistributionineellseanbecolleetedalltogetheratthesametimebythismethod.Inourst