辅酶q10的发酵工艺与

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1、.目录前言..................................................................11菌种的选育..........................................................21.1菌种的选育及制备................................................21.2诱变育种........................................................31

2、.3菌种的保藏......................................................52培养基的设计及灭菌..................................................62.1培养基的类型及功能..............................................62.2培养基的成分....................................................62.3培养基的设计及优化......

3、......................................72.4培养基设计时注意的问题..........................................82.5培养基灭菌......................................................83空气灭菌............................................................93.1过滤除菌流程及设备.........................

4、.....................93.2无菌空气的检查.................................................113.3发酵罐灭菌.....................................................114种子的扩大培养.....................................................124.1实验室种子制备...........................................

5、......124.2生产车间种子制备...............................................125发酵过程的参数控制.................................................135.1发酵过程的补料策略.............................................135.2发酵过程pH值的控制............................................135.3发酵过程温度的控制...

6、..........................................135.4溶氧的控制.....................................................145.5最佳接种量.....................................................145.6泡沫的影响及控制...............................................145.7染菌的控制........................

7、.............................146下游加工...........................................................15-..6.1发酵液的预处理和固液分离.......................................156.2细胞破碎及其碎片的分离.........................................156.3辅酶Q10的提取与纯化...................................

8、........157物料衡算...........................................................168发酵罐的设计.......................................................178.1发酵罐的结构.................