肠源性高草酸尿大鼠模型的建立与乳双歧杆菌对高草酸尿抑制作用的可行性分析

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1、-广州医科大学硕士学位论文肠源性高草酸尿大鼠模型的建立及乳双歧杆菌对高草酸尿抑制作用的可行性研究ToestablishamodelofEnterogenoushyperoxaluriainratsandafeasibilitystudyofBifidobacteriumlactisforhyperoxaluriainhibitionMajor：UrologyPostgraduate:QingjiangChenTutor:Prof.XunLiAbstractObject:ToestablishamodelofEnt

2、erogenoushyperoxaluriainrats.Bifidobacteriumlactisforhyperoxaluriainterventioneffectevaluation，topreventingandtreatingofurinarystonesMethods:Arandomlyselected30femaleSprague-Daw-leyratsinindividualmetaboliccageadaptivefeeding7days,respectively.respectively．Tw

3、osuccessive24hurinewascollectedtOmeasurethe24hurineoxalateexcretionbyionchromotagraphy.Theratswerelavagedbyspinachjuice.Twosuccessive24hurinewascollectedtOmeasurethe24hurineoxalateexcretionbyionchromotagraphyagain.Contrastbeforeandafterthelavagingspinachjuice

4、,the24hurineoxalateexcretionisthereanydifference;Inagroupcontrolexperiment,20femaleSprague-Daw-leyratswererandomlydividedintoAgroupandBgroup.BgroupwerelavagedbyspinachjuiceandAgroupwerelavagedequivalentpurewaterinthesameexperimentcondition.24hurineoxalateexcr

5、etionwerecomparedinAgroupandBgroup.BuildthefoundationforsuccessinaratmodelofEnterogenoushyperoxaluria,UsinganaerobicbagstocultureBifidobacteriumlactistomadeintoacoldsuspensionforgavagingEnterogenoushyperoxaluriainrats,Theintestinalhyperoxaluriaratswererandoml

6、ydividedintothreegroups1,2and3,n=20;Group1weregavagingspinachjuiceandinactivatedBifidobacteriumlactissuspension;Group2weregavaging------4分析------广州医科大学硕士学位论文肠源性高草酸尿大鼠模型的建立及乳双歧杆菌对高草酸尿抑制作用的可行性研究spinachjuiceandactivatedBifidobacteriumlactissuspension;Group3wereg

7、avagingspinachjuiceandthesameamountofpurewater;Respectivelydetectingthe24hexcretionofurinaryoxalateafterBifidobacteriumlactisinterventionin1d3d7dand10d,andrespectivelydetectingthe24hexcretionofurinaryoxalateafterstoppingBifidobacteriumlactisinterventionin10da

8、nd20d.Results:The24hurineoxalateexcretionwere（0.53±0.18）mgbeforelavagingspinachjuice.The24hurineoxalateexcretionwere（1.93±0.36）mg,（1.85±0.38）mg,（2.03±0.39）mgafterlavagingspinachjuiceinfri