伊犁马dmrt3 mstn actn3基因多态性与运动性能的相关性分析

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1、CorrelationAnalysisofPolymorphismsofDMRT3,MSTNandACTN3GeneswithSpotsPerfomanceinYiliHorseAbtractInthisstudy．Yilihorseswereusedforthemainexperimentobject．D．^组乃，』淄7ⅣandACTN3genewerechosenasmaincandidategenes．GeneticpolymorphismsweredetectedbyPCR-RFLP．PCR-SSCPandDNAsequen

2、cingtechnique．SelectedthegeneofracingperformanceinYilihorses，foundtheassociationwithDMRT3．MslWandACTN3genesonthesportsperformance．Providebasisfotpreliminarystudiesandtechnica、supportforfurtherexplorationofthegeneticmechanismofsportsperformanceYilihorsebreedsfromthemole

3、cularlevel．Theresultswereasfollows：1．CorrelationanalysisDMRT3geneticpolymorphismwithgaittraitsinYilihorseGeneticpolymorphismdetectiononexon1ofJDjI很T3geneindifferenttypesofYilihorse．whereD懈乃genepolymorphismininYilihorsewasthefirsttimetoreport．nestudyfoundthatthegenepoly

4、morphisminDMR乃，sequencingresultshowed也attheSNPwasg．22999655C>A．Itwasanonsensemutationtllatcreatedaprematurestopcodonthatresultsinatruncatedprotein．Therewerethreegenotypes：CC，CAandAA，Cwasthedominantallele．Afterassociationanalysis，Speedofpacingtypewasfasterthantrottingty

5、pe(P

6、ilihorseGeneticpolymorphismdetectiononintron1ofMSTNgenein4groupsofYilihorse．ThestudyfoundthatthegenepolymorphisminMSTN,sequencingresultshowedthattheSNPwasg．66493737C>T．Therewerethreegenotypes：CC，CTandTT，Cwasthedominantallelein2kmand15kmgroup，whileTwasthedominantallelei

7、n3kmand5kmgroup．Afterassociationanalysis，in5kmgroup，thismutationhadasignificanteffect(P

8、Yilihorse．Therewasnopolymorphismsin10，11and11exonsofthegene．Keyword：Yilihorse，sportsperformance，DMRT3，MSTN，ACTN3，poly