局部应用苯扎氯铵诱导的c57bl6小鼠干眼模型

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1、摘要各组小鼠眼表及淋巴结中均有免疫炎症的改变，且炎症因子IFN—Y(Thl相关)、IL一6(Thl7相关)、IL一17(Thl7相关)表达升高，IL．13(T112相关)表达降低。结论：研究发现在稳定的正常环境(温度23±1℃，相对湿度45±5％)条件下，使用浓度为O．075％的BAC溶液每天滴眼两次(9am，9pm)，连续7天，可成功诱导C57BL／6小鼠干眼模型。此模型能够很好模拟干眼病人的眼表体征，且免疫炎症改变也与干眼病人相似。通过局部用药诱导不易产生全身副作用、诱导方法简单且诱导周期短易推广，因此可以用于干眼病理机制的研究以及干眼治疗新药物的评估等。关键词：干眼；苯扎氯铵；小鼠

2、模型；炎症II万方数据AbstractAbstractobjective：ToexploretheoptimalconcentrationofBenzalkoniumChlorideusedintheinductionofdryeyemodelinC57BL／6mice，andtodetectedtheinflammatorychangesintheocularsurfaceandcervicallymphnodesinthismurinedryeyemodelMethods：TheexperimentalC57BL／6micewererandomlydividedintosixgrou

3、pscontrolgroupwhichreceivedvehiclecontroleyedrops．0．05％BACmicewhichreceived0．05％BACeyedrops，O．075％BACmicewhichreceivedO．075％BACeyedrops，O．1％BACmicewhichreceivedO．1％BACeyedrops，O．15％BACmicewhichreceived0．15％BACeyedropsand0．2％BACmicewhichreceived0．1％BACeyedrops．Allthemicewerehousedinastableenvironm

4、ent(temperatureof23-4-1℃，relativehumidityof45士5％)TopicalapplicationofBAKorvehicleeyedropswasperformedtwiceaday(9amand9pm)for7days．Thetearproductioninmicewasassessesbyphenolredcoaomtestafter7days．ThecornealbarrierfunctionofmousewasassessedbyOGDstainingImmunofluorescencestainingwasusedtoevaluateapo

5、ptosis，proliferation，squamousmetaplasiaandMMPSinocularsurface．Thenumberofmiceconjunctivalgobletcellswastestedbyperiodicacid-Schiff(periodicacid—Schiff,PAS)staining．Inaddition，real—timequantitativePCR(quantitativeReal—timePCR)wasusedtotesttheexpressionlevelsofinflammatorycytokinessuchasTNF—a，IFN吖，

6、IL一17，IL-6，IL-13andIL一113intheconjunctivaandcervicallymphnodesResults：Thisstudyshowedthat，whentheconcentrationofBAChigherthanorequalto0．1％．cornealneovascularizationand／orcornealulcersappearedalargeproportionofC57BL／6micef2eyesin10eyesof5micein0．1％BACgroupand6eyesin10eyesof5miceinO．2％BACgroup)．Dec

7、reasedtearsecretionwasnotedinO．05％BACgroup．0．075％BACgroupand0．1％BACgroup．butthedifferencewassignificantonlyinO．075％groupcomparedtothecontrolgroup．OGDstainingshowedthatthefluorescenceintensitywassignificantlyincreasedin